Automated capture and on-column detection of biotinylated DNA on a disposable solid support
| Autor: | Michael Decuir, Ilkka Lähdesmäki, Jaromir Ruzicka, Andrea D. Carroll |
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| Rok vydání: | 2007 |
| Předmět: |
On column
Biotin DNA Single-Stranded Bead Nucleic Acid Denaturation Biochemistry Analytical Chemistry chemistry.chemical_compound Electrochemistry Humans Environmental Chemistry Spectroscopy Fluorescent Dyes Detection limit Autoanalysis Chromatography Microfluidic Analytical Techniques Fluorescence Sequential injection chemistry Biotinylation visual_art Calibration Flow Injection Analysis visual_art.visual_art_medium Nanoparticles Agarose DNA |
| Zdroj: | The Analyst. 132:818-822 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | This work comprises the development of a technique for the capture of single-stranded DNA on a solid support combined with in situ quantification. The capture is based on the strong and selective interaction between biotinylated DNA and streptavidin-coated agarose beads. Sequential Injection in the lab-on-valve format allows for automated manipulation of all components including the building and disposal of bead columns. Detection was accomplished using the OliGreen fluorescent dye and optimization of the assay achieved a limit of detection of 111 pg ssDNA, with a total assay time of roughly 2.5 min per sample. |
| Databáze: | OpenAIRE |
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