The decarboxylation of the weak-acid preservative, sorbic acid, is encoded by linked genes in Aspergillus spp
| Autor: | Paul S. Dyer, Andrew Plumridge, Johannes Andries Roubos, J. Stark, David B. Archer, Lee Shunburne, Malcolm Stratford, Michaela Novodvorska, Petter Melin, Hein Stam, Hildegard Henna Menke |
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| Rok vydání: | 2010 |
| Předmět: |
Antifungal Agents
Carboxy-lyases Carboxy-Lyases Decarboxylation Genes Fungal Molecular Sequence Data Parabens Microbial Sensitivity Tests Synteny Microbiology Saccharomyces Fungal Proteins chemistry.chemical_compound Pentanes Gene Order Genetics Amino Acid Sequence Gene chemistry.chemical_classification Aspergillus biology Aspergillus niger biology.organism_classification Sorbic Acid Alkadienes Enzyme chemistry Biochemistry Multigene Family Sorbic acid Sequence Alignment Metabolic Networks and Pathways Transcription Factors |
| Zdroj: | Fungal Genetics and Biology. 47:683-692 |
| ISSN: | 1087-1845 |
| DOI: | 10.1016/j.fgb.2010.04.011 |
| Popis: | The ability to resist anti-microbial compounds is of key evolutionary benefit to microorganisms. Aspergillus niger has previously been shown to require the activity of a phenylacrylic acid decarboxylase (encoded by padA1) for the decarboxylation of the weak-acid preservative sorbic acid (2,4-hexadienoic acid) to 1,3-pentadiene. It is now shown that this decarboxylation process also requires the activity of a putative 4-hydroxybenzoic acid (3-octaprenyl-4-hydroxybenzoic acid) decarboxylase, encoded by a gene termed ohbA1, and a putative transcription factor, sorbic acid decarboxylase regulator, encoded by sdrA. The padA1,ohbA1 and sdrA genes are in close proximity to each other on chromosome 6 in the A. niger genome and further bioinformatic analysis revealed conserved synteny at this locus in several Aspergillus species and other ascomycete fungi indicating clustering of metabolic function. This cluster is absent from the genomes of A. fumigatus and A. clavatus and, as a consequence, neither species is capable of decarboxylating sorbic acid. |
| Databáze: | OpenAIRE |
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