A reconfigured Kennedy pathway which promotes efficient accumulation of medium-chain fatty acids in leaf oils

Autor: Matthew C. Taylor, Kyle Reynolds, James Robertson Petrie, Darren P. Cullerne, Surinder P. Singh, Christopher Blanchard, Craig C. Wood
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Cocos
Cinnamomum camphora
Arabidopsis
Plant Science
Arecaceae
Elaeis guineensis
01 natural sciences
chemistry.chemical_compound
Gene Expression Regulation
Plant
Biomass
Research Articles
chemistry.chemical_classification
Biodiesel
Chlorosis
biology
Cell Death
Fatty Acids
food and beverages
Lauric Acids
Plants
Genetically Modified
Biochemistry
Cocos nucifera
lipids (amino acids
peptides
and proteins)
triacylglycerol
Biotechnology
Research Article
Nicotiana
Membrane lipids
GPAT9
DGAT
03 medical and health sciences
Membrane Lipids
Tobacco
Plant Oils
Diacylglycerol O-Acyltransferase
Triglycerides
plant biomass
Fatty acid
biology.organism_classification
Lipid Metabolism
Lauric acid
Plant Leaves
030104 developmental biology
chemistry
fatty acid
Transcriptome
Agronomy and Crop Science
medium chain
010606 plant biology & botany
WRINKLED
Zdroj: Plant Biotechnology Journal
ISSN: 1467-7652
Popis: Summary Medium‐chain fatty acids (MCFA, C6‐14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, although these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer nonseed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death. Here we demonstrate that the introduction of a diacylglycerol acyltransferase from oil palm, EgDGAT1, was necessary to channel nascent MCFA directly into leaf oils and therefore bypassing MCFA residing in membrane lipids. This pathway resulted in increased flux towards MCFA rich leaf oils, reduced MCFA in leaf membrane lipids and, crucially, the alleviation of chlorosis. Deep sequencing of African oil palm (Elaeis guineensis) and coconut palm (Cocos nucifera) generated candidate genes of interest, which were then tested for their ability to improve oil accumulation. Thioesterases were explored for the production of lauric acid (C12:0) and myristic (C14:0). The thioesterases from Umbellularia californica and Cinnamomum camphora produced a total of 52% C12:0 and 40% C14:0, respectively, in transient leaf assays. This study demonstrated that the introduction of a complete acyl‐CoA‐dependent pathway for the synthesis of MFCA‐rich oils avoided disturbing membrane homoeostasis and cell death phenotypes. This study outlines a transgenic strategy for the engineering of biomass crops with high levels of MCFA rich leaf oils.
Databáze: OpenAIRE
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