Bactericidal Specificity and Resistance Profile of Poly(Quaternary Ammonium) Polymers and Protein-Poly(Quaternary Ammonium) Conjugates
| Autor: | Weihang Ji, Alan J. Russell, Hironobu Murata, Alan Campbell, Sawyer Zadan, Richard R. Koepsel |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Biocide
Polymers and Plastics Polymers Bioengineering Serum Albumin Human 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Polymer chemistry Materials Chemistry Copolymer Humans Ammonium chemistry.chemical_classification biology Bacteria Atom-transfer radical-polymerization Polymer 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Anti-Bacterial Agents Quaternary Ammonium Compounds HEK293 Cells chemistry Covalent bond Adsorption 0210 nano-technology Conjugate |
| Zdroj: | Biomacromolecules. 18(8) |
| ISSN: | 1526-4602 |
| Popis: | Antibacterial polymers are potentially powerful biocides that can destroy bacteria on contact. Debate in the literature has surrounded the mechanism of action of polymeric biocides and the propensity for bacteria to develop resistance to them. There has been particular interest in whether surfaces with covalently coupled polymeric biocides have the same mechanism of action and resistance profile as similar soluble polymeric biocides. We designed and synthesized a series of poly(quaternary ammonium) polymers, with tailorable molecular structures and architectures, to engineer their antibacterial specificity and their ability to delay the development of bacterial resistance. These linear poly(quaternary ammonium) homopolymers and block copolymers, generated using atom transfer radical polymerization, had structure-dependent antibacterial specificity toward Gram positive and negative bacterial species. When single block copolymers contained two polymer segments of differing antibacterial specificity, the polymer combined the specificities of its two components. Nanoparticulate human serum albumin-poly(quaternary ammonium) conjugates of these same polymers, synthesized via "grafting from" atom transfer radical polymerization, were strongly biocidal and also exhibited a marked decrease in the rate of bacterial resistance development relative to linear polymers. These protein-biocide conjugates mimicked the behavior of surface-presented polycationic biocides rather than their nonproteinaceous counterparts. |
| Databáze: | OpenAIRE |
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