The role of galectin-3 and its genetic variants in tumor risk and survival of patients with surgically resected early-stage non-small cell lung cancer
| Autor: | Akif Turna, Ilhan Yaylim, Şule Terzioğlu-Uşak, Cem Horozoglu, Seyda Demirkol |
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| Přispěvatelé: | Mühendislik ve Doğa Bilimleri Fakültesi, TERZİOĞLU, ŞULE |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine medicine.medical_specialty Tek Nükleotid Polimorfizm Genotiplemesi Küçük Hücreli Dışı Akciğer Kanseri Gene Expression Single-nucleotide polymorphism Enzyme-Linked Immunosorbent Assay Gastroenterology 03 medical and health sciences 0302 clinical medicine Enzyme-linked immunosorbent assay Enzim Bağlı İmmünosorbent Testi single nucleotide polymorphism genotyping Internal medicine galectin-3 Genotype medicine Galectin-3 Lung cancer non-small cell lung cancer Univariate analysis Predictive marker business.industry Hazard ratio Area under the curve Prognosis medicine.disease Non-Small Cell Lung Cancer Gen Ekspresyonu 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis Single Nucleotide Polymorphism Genotyping Galektin-3 gene expression Surgery Original Article Terzioglu-Usak S. Horozoglu C. Demirkol S. Turna A. Yaylim I. -The role of galectin-3 and its genetic variants in tumor risk and survival of patients with surgically resected early-stage non-small cell lung cancer- TURK GOGUS KALP DAMAR CERRAHISI DERGISI-TURKISH JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY cilt.29 sa.2 ss.212-222 2021 Cardiology and Cardiovascular Medicine business |
| Zdroj: | Turkish Journal of Thoracic and Cardiovascular Surgery |
| Popis: | Background: The aim of this study was to investigate the possible relationship between galectin-3 gene variants, serum level, gene expression level, and the risks and survivals of resectable non-small cell lung cancer patients. Methods: The rs4644 and rs4652 variants of galectin-3 were genotyped by TaqMan single nucleotide polymorphism assay using genomic deoxyribonucleic acid isolated from the peripheral blood of 65 (54 males, 11 females; mean age: 60.1±11.9 years; range, 34 to 83 years) with Stage IA-IIIA non-small cell lung cancer who underwent primary surgical treatment and 95 healthy individuals (48 males, 47 females; mean age: 53.9±13.5 years; range, 32 to 87 years) between March 2017 and September 2018. Circulating galectin-3 levels in serum samples of the patient and control groups were assessed by enzyme-linked immunosorbent assay. Messenger ribonucleic acid expression of galectin-3 in tumor and surrounding tissues of the patient group was examined by real-time quantitative polymerase chain reaction. Both predictive and prognostic significance of the results were analyzed. Results: The presence of angiolymphatic invasion was significant in the patients with rs4652 AA genotype (p=0.04). Serum galectin-3 levels were significantly higher in the patients than the controls (p Amaç: Bu çalışmada galektin-3 gen varyantları, serum düzeyi, gen ekspresyon düzeyi ile rezektabl küçük hücreli dışı akciğer kanseri hastalarının riskleri ve sağkalımları arasındaki olası ilişki araştırıldı. Çalışma planı: Mart 2017 - Eylül 2018 tarihleri arasında galektin-3'ün rs4644 ve rs4652 varyantları, primer cerrahi tedavi yapılan küçük hücreli dışı akciğer kanserli 65 hastanın (54 erkek, 11 kadın; ort. yaş: 60.1±11.9 yıl; dağılım, 34-83 yıl) ve 95 sağlıklı bireyin (48 erkek, 47 kadın; ort. yaş: 53.9±13.5 yıl; dağılım, 32-87 yıl) periferik kanından izole edilen genomik deoksiribonükleik asitler kullanılarak TaqMan tek nükleotid polimorfizm testi ile genotiplendi. Hasta ve kontrol gruplarının serum örneklerinde dolaşımdaki galektin-3 düzeyi, enzim bağlı immünosorbent testi ile değerlendirildi. Hasta grubunda tümör ve çevre dokulardaki galektin-3’ün haberci ribonükleik asit ekspresyonu, gerçek zamanlı kantitatif polimeraz zincir reaksiyonu ile incelendi. Bu sonuçların hem prediktif, hem de prognostik önemi analiz edildi. Bulgular: Rs4652 AA genotipi olan hastalarda anjiyolenfatik invazyon varlığı anlamlı idi (p=0.04). Kontrol grubuna kıyasla, hastalarda serum galektin-3 düzeyleri anlamlı düzeyde yüksekti (p |
| Databáze: | OpenAIRE |
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