Insulin Processing and Dissociation - Effect of Temperature and Lysosomotropic Agents
| Autor: | Ludeman C, Sherrod J, Gebhart Sp, Blackard Wg |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Kinetics Biochemistry Dissociation (chemistry) Methylamines chemistry.chemical_compound Endocrinology Chloroquine Internal medicine medicine Animals Insulin Insulin degradation Cells Cultured Methylamine Biochemistry (medical) Temperature Rats Inbred Strains General Medicine Rats Liver chemistry Biophysics Insulin processing medicine.drug |
| Zdroj: | Hormone and Metabolic Research. 16:298-302 |
| ISSN: | 1439-4286 0018-5043 |
| DOI: | 10.1055/s-2007-1014773 |
| Popis: | Dissociation of intact and degraded insulin from hepatocytes in monolayer culture was examined under conditions in which processing of insulin was altered by either temperature or pharmacologic agents. Conditions which increased insulin degradation or processing decreased equilibrium insulin binding whereas those conditions which inhibited processing increased equilibrium binding. The effect of lysosomotropic agents on processing was markedly temperature dependent. Not only was processing increased at higher temperatures (37 degrees C, but the effect of lysosomal inhibitors (chloroquine and methylamine) on insulin processing was abolished at this temperature. The temperature dependency of this effect may explain discrepancies between laboratories on the effect of these inhibitors in hepatocytes. |
| Databáze: | OpenAIRE |
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