TLR4 Asp299Gly and Thr399Ile and TLR2 intron 2 microsatellite gene polymorphism in patients with acute biliary pancreatitis: Does it cause the disease?

Autor:	Filiz Ozen, Orhan Alimoglu, Çağrı Bilgiç, Ibrahim Halil Yildirim, Ender Anilir, Ibrahim Ali Ozemir
Rok vydání:	2018
Předmět:	medicine.medical_specialty business.industry medicine.disease Gastroenterology TLR2 Internal medicine Genotype TLR4 Medicine Microsatellite Acute pancreatitis Original Article Gene polymorphism Restriction fragment length polymorphism business Receptor
Zdroj:	Turkish Journal of Surgery. 34:191-197
ISSN:	2564-7032 2564-6850
DOI:	10.5152/turkjsurg.2017.3828
Popis:	OBJECTIVES There has been coverage of Toll-like receptor 4 and Toll-like receptor 2 gene polymorphisms in inflammatory episodes in a number of studies. In view of the inflammatory nature of acute pancreatitis, we aimed to determine the predictive value of mutations in Asp299Gly and Thr399Ile of the Toll-like receptor 4 gene, and the intron 2 microsatellite polymorphism of the Toll-like receptor 2 gene on the occurrence of acute biliary pancreatitis. MATERIAL AND METHODS The study included 86 patients for the Toll-like receptor 4 Thr399Ile polymorphism, 100 patients for the Toll-like receptor 4 Asp299Gly polymorphism with acute biliary pancreatitis, and 101 healthy volunteers. At the same time, 93 patients and 92 healthy volunteers were included in the study to research the Toll-like receptor 2 intron 2 microsatellite polymorphism. Genotypes were determined using the restriction fragment length polymorphism analysis of PCR products and by an allele-specific PCR. RESULTS The Toll-like receptor 4 Thr399Ile homozygotes mutant variants (p=0.005) and Toll-like receptor 2 MM genotype (p
Databáze:	OpenAIRE
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