External Quality Assessment (EQA) program for the preanalytical and analytical immunohistochemical determination of HER2 in breast cancer: an experience on a regional scale
| Autor: | Paola Pasquini, Irene Terrenato, Letizia Perracchio, Elena Bonanno, Marcella Mottolese, Ilaria Pennacchia, Maria Nicoletta Pericoli, Anna Crescenzi, Leopoldo Costarelli, Vincenzo Arena, Stefania Scarpino, Luigi Ruco, Domenico Vitolo, Domenica Di Stefano, Maria Teresa Ramieri, Francesca Sperati, Sara Pizzamiglio, Daniela Baldini, Antonella Dal Mas, Vito Gomes, Simonetta Buglioni, Giulia d'Amati, Paolo Verderio, Claudio Di Cristofano, Cristiana Ercolani, Silvia Candia, Lucia Rosalba Grillo, Angelo Paradiso |
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| Rok vydání: | 2013 |
| Předmět: |
Questionnaires
Quality Control Reproducibility of results Receptor erbB-2/analysis Cancer Research medicine.medical_specialty Receptor ErbB-2 Concordance Breast Neoplasms Settore MED/08 - Anatomia Patologica Breast Neoplasm/drug therapy Cohen's kappa Surveys and Questionnaires Immunohistochemistry/methods External quality assessment medicine Humans Medical physics immunohistochemistry/ methods Statistic erbB-2 Gynecology Reproducibility business.industry Research Reproducibility of Results Receptor erbB-2 Immunohistochemistry Female Confidence interval reproducibility of results quality control breast neoplasm/drug therapy receptor erbb-2/analysis Oncology business Jackknife resampling Kappa Receptor |
| Zdroj: | Journal of Experimental & Clinical Cancer Research : CR |
| ISSN: | 1756-9966 |
| Popis: | Background An External Quality Assessment (EQA) program was developed to investigate the state of the art of HER2 immunohistochemical determination in breast cancer (BC) in 16 Pathology Departments in the Lazio Region (Italy). This program was implemented through two specific steps to evaluate HER2 staining (step 1) and interpretation (step 2) reproducibility among participants. Methods The management activities of this EQA program were assigned to the Coordinating Center (CC), the Revising Centers (RCs) and the Participating Centers (PCs). In step 1, 4 BC sections, selected by RCs, were stained by each PC using their own procedures. In step 2, each PC interpreted HER2 score in 10 BC sections stained by the CC. The concordance pattern was evaluated by using the kappa category-specific statistic and/or the weighted kappa statistic with the corresponding 95% Jackknife confidence interval. Results In step 1, a substantial/almost perfect agreement was reached between the PCs for scores 0 and 3+ whereas a moderate and fair agreement was observed for scores 1+ and 2+, respectively. In step 2, a fully satisfactory agreement was observed for 6 out of the 16 PCs and a quite satisfactory agreement was obtained for the remaining 10 PCs. Conclusions Our findings highlight that in the whole HER2 evaluation process the two intermediate categories, scores 1+ and 2+, are less reproducible than scores 0 and 3+. These findings are relevant in clinical practice where the choice of treatment is based on HER2 positivity, suggesting the need to share evaluation procedures within laboratories and implement educational programs. |
| Databáze: | OpenAIRE |
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