MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness
| Autor: | So Mee Kwon, Jiwon Hong, Seongki Min, Young-Kyoung Lee, Gyesoon Yoon, Tae Jun Park, Hyun Goo Woo |
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| Rok vydání: | 2021 |
| Předmět: |
Ribosomal Proteins
Cancer Research Carcinoma Hepatocellular Somatic cell Immunology Biology Transfection SCNA Autoantigens MMP7 Article Metastasis Tumour biomarkers Transcriptome Cellular and Molecular Neuroscience Biomarkers Tumor Cancer genomics medicine Humans DAP3 QH573-671 Liver Neoplasms Genomics Cell Biology medicine.disease Phenotype digestive system diseases Hepatocellular carcinoma Cancer cell Cancer research Cytology |
| Zdroj: | Cell Death and Disease, Vol 12, Iss 11, Pp 1-12 (2021) Cell Death & Disease |
| ISSN: | 2041-4889 |
| DOI: | 10.1038/s41419-021-04370-8 |
| Popis: | Abstract Deregulated mitochondrial energetics is a metabolic hallmark of cancer cells. However, the causative mechanism of the bioenergetic deregulation is not clear. In this study, we show that somatic copy number alteration (SCNA) of mitoribosomal protein (MRP) genes is a key mechanism of bioenergetic deregulation in hepatocellular carcinoma (HCC). Association analysis between the genomic and transcriptomic profiles of 82 MRPs using The Cancer Genome Atlas-Liver HCC database identified eight key SCNA-dependent MRPs: MRPS31, MRPL10, MRPL21, MRPL15, MRPL13, MRPL55, and DAP3. MRPS31 was the only downregulated MRP harboring a DNA copy number (DCN) loss. MRPS31 loss was associated specifically with the DCN losses of many genes on chromosome 13q. Survival analysis revealed a unique dependency of HCC on the MRPS31 deficiency, showing poor clinical outcome. Subclass prediction analysis using several public classifiers indicated that MRPS31 loss is linked to aggressive HCC phenotypes. By employing hepatoma cell lines with SCNA-dependent MRPS31 expression (JHH5, HepG2, Hep3B, and SNU449), we demonstrated that MRPS31 deficiency is the key mechanism, disturbing the whole mitoribosome assembly. MRPS31 suppression enhanced hepatoma cell invasiveness by augmenting MMP7 and COL1A1 expression. Unlike the action of MMP7 on extracellular matrix destruction, COL1A1 modulated invasiveness via the ZEB1-mediated epithelial-to-mesenchymal transition. Finally, MRPS31 expression further stratified the high COL1A1/DDR1-expressing HCC groups into high and low overall survival, indicating that MRPS31 loss is a promising prognostic marker. Significance Our results provide new mechanistic insight for mitochondrial deregulation in HCC and present MRPS31 as a novel biomarker of HCC malignancy. |
| Databáze: | OpenAIRE |
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