Reduction of Proteinuria through Podocyte Alkalinization
| Autor: | Mehmet M. Altintas, Mohd Hafeez Faridi, Jing Li, Clemens C. Möller, Kumiko Moriwaki, Tobias B. Huber, Richard A. Preston, Jan Flesche, Suma Yaddanapudi, Jochen Reiser, Changli Wei, Sanja Sever, Markus Gödel, Dontscho Kerjaschki, Jean X. Jiang |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
Intracellular pH medicine.medical_treatment Biological Transport Active urologic and male genital diseases Biochemistry Podocyte Cathepsin L Mice Internal medicine medicine Animals Molecular Biology Cells Cultured Cytoskeleton Mice Knockout Kidney Protease biology Podocytes fungi food and beverages Cell Biology Metabolism Hydrogen-Ion Concentration Glutamine Proteinuria Cytosol medicine.anatomical_structure Endocrinology biology.protein |
| Zdroj: | Journal of Biological Chemistry. 289:17454-17467 |
| ISSN: | 0021-9258 |
| Popis: | Podocytes are highly differentiated cells and critical elements for the filtration barrier of the kidney. Loss of their foot process (FP) architecture (FP effacement) results in urinary protein loss. Here we show a novel role for the neutral amino acid glutamine in structural and functional regulation of the kidney filtration barrier. Metabolic flux analysis of cultured podocytes using genetic, toxic, and immunologic injury models identified increased glutamine utilization pathways. We show that glutamine uptake is increased in diseased podocytes to couple nutrient support to increased demand during the disease state of FP effacement. This feature can be utilized to transport increased amounts of glutamine into damaged podocytes. The availability of glutamine determines the regulation of podocyte intracellular pH (pHi). Podocyte alkalinization reduces cytosolic cathepsin L protease activity and protects the podocyte cytoskeleton. Podocyte glutamine supplementation reduces proteinuria in LPS-treated mice, whereas acidification increases glomerular injury. In summary, our data provide a metabolic opportunity to combat urinary protein loss through modulation of podocyte amino acid utilization and pHi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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