Circadian gene expression patterns of melanopsin and pinopsin in the chick pineal gland
| Autor: | Heike Holthues, Rainer Spessert, Lutz Vollrath, Lydia Engel |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Melanopsin
medicine.medical_specialty Light Photoperiod Circadian clock Biophysics Nerve Tissue Proteins Biology Pineal Gland Biochemistry Avian Proteins Pineal gland Internal medicine Gene expression medicine Animals Photopigment Circadian rhythm Molecular Biology Cells Cultured Regulation of gene expression Adaptation Ocular Rod Opsins Cell Biology Circadian Rhythm medicine.anatomical_structure Endocrinology Animals Newborn Gene Expression Regulation Light effects on circadian rhythm sense organs Chickens |
| Zdroj: | Biochemical and Biophysical Research Communications. 326:160-165 |
| ISSN: | 0006-291X |
| Popis: | The directly light-sensitive chick pineal gland contains at least two photopigments. Pinopsin seems to mediate the acute inhibitory effect of light on melatonin synthesis, whereas melanopsin may act by phase-shifting the intrapineal circadian clock. In the present study we have investigated, by means of quantitative RT-PCR, the daily rhythm of photopigment gene expression as monitored by mRNA levels. Under a 12-h light/12-h dark cycle, the mRNA levels of both pigments were 5-fold higher in the transitional phase from light to dark than at night, both in vivo and in vitro. Under constant darkness in vivo and in vitro, the peak of pinopsin mRNA levels was attenuated, whereas that of melanopsin was not. Thus, whereas the daily rhythm of pinopsin gene expression is dually regulated by light plus the intrapineal circadian oscillator, that of melanopsin appears to depend solely on the oscillator. |
| Databáze: | OpenAIRE |
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