Comprehensive profiling of ribonucleosides modification by affinity zirconium oxide-silica composite monolithic column online solid–phase microextraction – Mass spectrometry analysis
| Autor: | Na Yang, Yu-Qi Feng, Han-Peng Jiang, Ping Liu, Bi-Feng Yuan, Fang Zheng, Meng-Dan Lan, Jie-Mei Chu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Monolithic HPLC column Silicon dioxide Ribose Urine Tandem mass spectrometry Mass spectrometry Solid-phase microextraction 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Neoplasms Humans Chromatography High Pressure Liquid Solid Phase Microextraction Chromatography 010401 analytical chemistry Organic Chemistry General Medicine Hydrogen-Ion Concentration Silicon Dioxide 0104 chemical sciences 030104 developmental biology chemistry Case-Control Studies Ribonucleosides Zirconium Conjugate |
| Zdroj: | Journal of Chromatography A. 1462:90-99 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2016.07.086 |
| Popis: | More than 140 modified ribonucleosides have been identified in RNA. Determination of endogenous modified ribonucleosides in biological fluids may serve as non-invasive disease diagnostic strategy. However, detection of the modified ribonucleosides in biological fluids is challenging, especially for the low abundant modified ribonucleosides due to the serious matrix interferences of biological fluids. Here, we developed a facile preparation strategy and successfully synthesized zirconium oxide-silica (ZrO2/SiO2) composite capillary monolithic column that exhibited excellent performance for the selective enrichment of cis-diol-containing compounds. Compared with the boronate-based affinity monolith, the ZrO2/SiO2 monolith showed ∼2 orders of magnitude higher extraction capacity and can be used under physiological pH (pH 6.5-7.5). Using the prepared ZrO2/SiO2 composite monolith as the trapping column and reversed-phase C18 column as the analytical column, we further established an online solid-phase microextraction (SPME) in combination with liquid chromatography-mass spectrometry (online SPME-LC-MS/MS) analysis for the comprehensive profiling of ribonucleosides modification in human urine. Our results showed that 68 cis-diol-containing ribosylated compounds were identified in human urine, which is, to the best of our knowledge, the highest numbers of cis-diol-containing compounds were determined in a single analysis. It is worth noting that four modified ribonucleosides were discovered in the human urine for the first time. In addition, the quantification results from the pooled urine samples showed that compared to healthy controls, the contents of sixteen ribose conjugates in the urine of gastric cancer, eleven in esophagus cancer and seven in lymphoma increased more than two folds. Among these ribose conjugates, four ribose conjugates increased more than two folds in both gastric cancer and esophagus cancer; three ribose conjugates increased more than two folds in both gastric cancer and lymphoma; one ribose conjugate increased more than two folds in both esophagus cancer and lymphoma. The developed analytical method provides a good platform to study the modified ribonucleosides in human body fluids. |
| Databáze: | OpenAIRE |
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