Herpes Simplex Virus Type I Infection of Mice Testis and Effect on Fertility

Autor: Naumenko, Victor A., Tyulenev, Yuriy A., Kushch, Alla A.
Jazyk: angličtina
Rok vydání: 2012
Předmět:
DOI: 10.5281/zenodo.1056128
Popis: The objective of current issue was to develop a model of testicular herpes simplex virus (HSV) type I infection for assessment of viral effect on fertility. 56 male mice were inoculated intraperitoneally with different concentrations of HSV on 8 day post partum. It was revealed that the optimal dose was 100 plaque forming units per mice as it provided testicular infection in 100% of survivors. HSV proteins were detected both in somatic and germ cells (spermatogonia, spermatocytes, spermatides). Although DNA load in testis was descending from 3 to 28 days post infection only 12.5% of infected males had offspring after mating with uninfected females comparing to 87.5% in control (p=0.012). These results are the first direct evidence for HSV impact in male sterility. Prepuberal mice appeared to be a suitable model for investigation of pathogenesis of virus-associated fertility disorders.
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