Validation of the Applied Biosystems 7500 Fast Instrument for Detection of Listeria monocytogenes with the SureTect Listeria monocytogenes PCR Assay
| Autor: | Suvi Koskela, Laura Vaahtoranta, Jukka-Pekka Palomäki, Paulus Artimo, Feng Huang, Maria Liikanen, Katharine Evans, Jonathan Cloke, Helen Simpson, David Crabtree, Julia Arizanova |
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| Rok vydání: | 2016 |
| Předmět: |
Pharmacology
Validation study Bacteriological Techniques Chromatography Significant difference Pcr assay Fast pcr medicine.disease_cause Real-Time Polymerase Chain Reaction Listeria monocytogenes Analytical Chemistry Microbiology Ground turkey medicine Environmental Chemistry Statistical analysis Agronomy and Crop Science Food Science Mathematics |
| Zdroj: | Journal of AOAC International. 99(3) |
| ISSN: | 1944-7922 |
| Popis: | In 2013, the Thermo Scientific™ SureTect™ Listeria monocytogenes Real-Time PCR Assay was certified by the AOAC Research Institute (RI) Performance Tested MethodsSM program as a rapid method for the detection of L. monocytogenes from a wide range of food matrixes and surface samples. This report details the method modification studies undertaken to extend the analysis of this PCR assay to the Applied Biosystems™ 7500 Fast PCR Instrument and Applied Biosystems RapidFinder™ Express 2.0 software allowing the use of the SureTect assay on a 96 well format PCR cycler in addition to the current workflow, which uses the 24 well Thermo Scientific PikoReal™ PCR Instrument and Thermo Scientific SureTect software. Because this study was deemed by AOAC-RI to be a level 2 method modification study, a representative range of food matrixes covering raw ground turkey, 2% fat pasteurized milk, and bagged lettuce as well as stainless steel surface samples were analyzed with the Applied Biosystems 7500 Fast PCR Instrument and RapidFinder Express 2.0 software. All testing was conducted in comparison to the reference method detailed in International Organization for Standardization (ISO) 6579:2002. No significant difference by probability of detection statistical analysis was found between the SureTect Listeria monocytogenes PCR Assay or the ISO reference method methods for any of the matrixes analyzed during the study. |
| Databáze: | OpenAIRE |
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