Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector
| Autor: | Xifeng Wang, Marcia Beatriz Moya Fernández, Lu Zhang, Jamal-U-Ddin Hajano, Wenwen Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine viruses Biology 01 natural sciences Microbiology Article Plant Viruses 03 medical and health sciences Rice black-streaked dwarf virus Crop production Virology Plant virus rice stripe virus (RSV) Animals Intestinal Mucosa virus interaction Rice plant virus diseases food and beverages acquisition Rice stripe virus respiratory system biology.organism_classification QR1-502 Insect Vectors Titer 030104 developmental biology Infectious Diseases Vector (epidemiology) Microbial Interactions Brown planthopper rice black-streaked dwarf virus (RBSDV) accumulation 010606 plant biology & botany |
| Zdroj: | Viruses Volume 13 Issue 6 Viruses, Vol 13, Iss 1121, p 1121 (2021) |
| ISSN: | 1999-4915 |
| Popis: | Plant viruses transmitted by hemipteran vectors commonly cause losses to crop production. Rice stripe virus (RSV) and rice black streaked dwarf virus (RBSDV) are transmitted to rice plants by the same vector, the small brown planthopper (SBPH), Laodelphax striatellus Fallén, in a persistent propagative manner. However, rarely do the respective diseases they cause occur simultaneously in a field. Here, we determined the acquisition efficiency of RSV and RBSDV when acquired in succession or simultaneously by SBPH. When RBSDV was acquired first, RSV acquisition efficiency was significantly lower than when only acquiring RSV. However, RBSDV acquisition efficiency from insects that acquired RSV first was not significantly different between the insects only acquiring RBSDV. Immunofluorescence assays showed that the acquisition of RBSDV first might inhibit RSV entry into midgut epithelial cells, but RSV did not affect RBSDV entry. SBPHs were more likely to acquire RBSDV when they were feeding on plants coinfected with the two viruses. When RBSDV was acquired before RSV, RBSDV titer was significantly higher and RSV titer first declined, then increased compared to when only acquiring RBSDV or RSV. Only 5% of the SBPHs acquired both viruses when feeding on plants coinfected with RSV and RBSDV. These results provide a better understanding of the interaction between two persistent viruses when present in the same vector insect and explain why RSV and RBSDV occur in intermittent epidemics. |
| Databáze: | OpenAIRE |
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