Laccase Immobilized Fe3O4-Graphene Oxide Nanobiocatalyst Improves Stability and Immobilization Efficiency in the Green Preparation of Sulfa Drugs
| Autor: | Rose Waithiegeni Kibechu, Titus A.M. Msagati, Bhekie B. Mamba, Shamila Rouhani, Shohreh Azizi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Contact time
Oxide 02 engineering and technology lcsh:Chemical technology 01 natural sciences Catalysis law.invention laccase lcsh:Chemistry chemistry.chemical_compound law sulfonamides lcsh:TP1-1185 Physical and Theoretical Chemistry Trametes versicolor Laccase magnetic nanobiocatalyst Nanocomposite Lac/Fe3O4/GO biology 010405 organic chemistry Graphene Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Enzyme assay 0104 chemical sciences lcsh:QD1-999 immobilization biology.protein Glutaraldehyde 0210 nano-technology Nuclear chemistry |
| Zdroj: | Catalysts, Vol 10, Iss 459, p 459 (2020) Catalysts Volume 10 Issue 4 |
| ISSN: | 2073-4344 |
| Popis: | This paper, reports on the novel and green synthesis procedure for sulfonamides that involved the immobilization of Trametes Versicolor laccase onto the Fe3O4&ndash graphene nanocomposite via glutaraldehyde (GA) crosslinking (Lac/Fe3O4/GO). Various parameters, mainly, activation time, GA, and laccase concentration were investigated and optimized. The results showed that the optimal contact time was 4 h, GA concentration was 5% while laccase concentration was 5 mg· mL&minus 1, at which a high enzyme activity recovery was achieved (86%). In terms of the stability of immobilized laccase to temperature and storage conditions, the performance of the nanobiocatalyst was found to significantly exceed that of free laccase. The results have indicated that nearly 70% of relative activity for immobilized laccase remained after the incubation period of 2 h at 55 ° C, but only 48% of free laccase remained within the same time period. Moreover, the immobilized laccase retained 88% of its initial activity after storage for 20 days. In case of the free laccase, the activity retained within the same time period was 32%. In addition, the nanobiocatalyst possessed better recycling performance as evidenced from the observation that after eight cycles of repeated use, it retained 85% of its original activity. |
| Databáze: | OpenAIRE |
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