DSC and Raman study of DMPC liposomes in presence of Ibuprofen at different pH
| Autor: | Anna Tinti, M. Di Foggia, Sergio Bonora, Vitaliano Tugnoli |
|---|---|
| Přispěvatelé: | Di Foggia, M, Bonora, S., Tinti, A., Tugnoli, V. |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Ibuprofen
02 engineering and technology Condensed Matter Physic 01 natural sciences DSC symbols.namesake Differential scanning calorimetry medicine Physical and Theoretical Chemistry Liposome Chromatography Chemistry Bilayer technology industry and agriculture Penetration (firestop) 021001 nanoscience & nanotechnology Condensed Matter Physics 010406 physical chemistry 0104 chemical sciences Membrane Raman spectroscopy symbols DMPC 0210 nano-technology Drug carrier Nuclear chemistry medicine.drug |
| Popis: | Ibuprofen (IbuH), 2-(4-isobutylphenyl) propionic acid, is a well-known nonsteroidal anti-inflammatory drug and is a promising antiatherosclerotic agent. Since IbuH has demonstrated to affect cell membranes structure, the study of the interaction between the membrane components, like phospholipids, and the drug, is of paramount interest. Moreover, liposomes can be used as drug carriers. In this paper, the effect of increasing amounts of Ibuprofen at neutral and acidic pH on the behaviour of dimyristoylphosphatidylcholine (DMPC) liposomes was investigated by means of Raman and differential scanning calorimetry (DSC) techniques. The results showed that pH influenced noticeably the liposome structure. A simple ‘solution-like’ model can explain the system when small or high IbuH or IbuNa (IbuH sodium salt forming at neutral and basic pH) amounts are present (IbuH/DMPC molar ratio ≤1/8.3 or ≥1/2.2 or IbuNa/DMPC molar ratio ≤1/17.5 or ≥1/4), whereas at intermediate amounts, two DSC peaks appeared: these systems could be described as a mixture of ‘phase II’ domains inserted within a structure of smaller and further ramified ‘phase I’ domains. Both DSC and Raman data suggested IbuH can penetrate within the apolar bilayer at pH 3, while at pH 7, the setting up of polar interactions of different strength between the carboxylic groups of IbuNa and the choline head of the DMPC, hamper a further penetration of the drug. Similar results were obtained both inserting directly IbuH molecules into liposomes as well as acidifying IbuNa water solutions, suggesting a possible use of liposomes as drug carriers when polarity is pH dependent. |
| Databáze: | OpenAIRE |
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