Brief fixation enables same-day breast cancer diagnosis with reliable assessment of hormone receptors, E-cadherin and HER2/Neu
| Autor: | J.P. Bulte, Peter Bult, Hans de Wilt, Iris D. Nagtegaal, Hanneke Braam, Oliver B. A. Boelens, Patricia van Cleef, Margrethe Schlooz-Vries, Annelies Werner, Yvonne Jacobs, Altuna Halilovic |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology Time Factors Tissue Fixation Receptor ErbB-2 medicine.medical_treatment HER2/neu Workflow Tumours of the digestive tract Radboud Institute for Health Sciences [Radboudumc 14] 0302 clinical medicine Tumours of the digestive tract Radboud Institute for Molecular Life Sciences [Radboudumc 14] skin and connective tissue diseases In Situ Hybridization Fluorescence Neoadjuvant therapy Netherlands Fixation (histology) Aged 80 and over Academic Medical Centers Women's cancers Radboud Institute for Molecular Life Sciences [Radboudumc 17] medicine.diagnostic_test biology General Medicine Middle Aged Cadherins Immunohistochemistry Receptors Estrogen Hormone receptor 030220 oncology & carcinogenesis Female Receptors Progesterone Adult medicine.medical_specialty Breast Neoplasms Pathology and Forensic Medicine 03 medical and health sciences Breast cancer Antigens CD Predictive Value of Tests Biopsy Progesterone receptor Biomarkers Tumor medicine Humans Aged business.industry Reproducibility of Results medicine.disease 030104 developmental biology biology.protein Biopsy Large-Core Needle business |
| Zdroj: | Journal of Clinical Pathology : the Journal of the Association of Clinical Pathologists, 70, 9, pp. 781-786 Journal of Clinical Pathology : the Journal of the Association of Clinical Pathologists, 70, 781-786 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jclinpath-2017-204362 |
| Popis: | AimsPreoperative core needle biopsy (CNB) is commonly used to confirm the diagnosis of breast cancer. For treatment purposes and for determining histological type, especially in case of neoadjuvant therapy, oestrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2) status and E-cadherin assessments are crucial. Considering the increasing demand for same-day diagnosis of breast lesions, an accelerated method of CNB processing was developed, in which the tissue fixation time is radically reduced.MethodsTo determine whether short fixation time frustrates assessment of ER, PR and E-cadherin immunohistochemistry (IHC) and HER2 fluorescence in situ hybridisation (FISH), 69 consecutive patients with 70 invasive breast carcinomas were included through the same-day diagnostics programme of breast lesions of the Radboud university medical center and the hospital Pantein. IHC for ER, PR and E-cadherin and HER2 FISH were compared between CNBs fixed for approximately 60–90 min and traditionally fixed resection specimens.ResultsOverall agreement between CNBs and resection specimens was 98.6% for ER (pConclusionsHormone receptors and E-cadherin IHC and HER2 FISH are highly comparable between briefly fixed CNBs and the corresponding traditionally fixed resection specimens, and can therefore reliably be used in the daily clinical practice of same-day diagnostics of breast cancer. |
| Databáze: | OpenAIRE |
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