Comparison of the Simplexa™ Flu A/B & RSV kit (nucleic acid extraction–dependent assay) and the Prodessa ProFlu+™ assay for detecting influenza and respiratory syncytial viruses
| Autor: | Amy Leber, Anami Patel, Suresh B. Selvaraju, Adrienne V. Bambach, Maria-Magdalena Patru, Marilyn A. Menegus |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Respiratory Syncytial Virus Infections Biology Sensitivity and Specificity Virus Young Adult Nasopharynx Influenza Human Humans Respiratory system Child Aged Retrospective Studies Aged 80 and over Infant virus diseases Influenza a General Medicine Middle Aged Virology Respiratory Syncytial Viruses Reverse transcription polymerase chain reaction Influenza B virus Infectious Diseases Molecular Diagnostic Techniques Influenza A virus Child Preschool Nucleic acid Female |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 80:50-52 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2013.11.015 |
| Popis: | The relative performance of 2 widely used reverse transcription polymerase chain reaction (RT-PCR) assays, the Focus diagnostics Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa Proflu+™ assay, was evaluated using 735 prospectively and retrospectively collected nasopharyngeal swab specimens. Overall, the assays showed positive and negative agreements of 100% and 99.7% for influenza A, 98.1% and 99.9% for influenza B, and 99.3% and 99.5% for respiratory syncytial virus. The relative analytical sensitivity of the 2 assays was also similar. |
| Databáze: | OpenAIRE |
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