Development of a lateral flow immunoassay of C-reactive protein detection based on red fluorescent nanoparticles
| Autor: | Xiaowei He, Yujia Liu, Yanxue Cai, Yu Wang, Keren Kang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
medicine.drug_class
Biophysics 02 engineering and technology Monoclonal antibody 01 natural sciences Biochemistry Chromatography Affinity Limit of Detection Oxazines medicine Humans Molecular Biology Detection limit Immunoassay Reproducibility Fluorescent nanoparticles Chromatography biology medicine.diagnostic_test Chemistry 010401 analytical chemistry C-reactive protein Antibodies Monoclonal Reproducibility of Results Cell Biology 021001 nanoscience & nanotechnology 0104 chemical sciences C-Reactive Protein biology.protein Nanoparticles 0210 nano-technology Quantitative analysis (chemistry) Conjugate |
| Zdroj: | Analytical biochemistry. 556 |
| ISSN: | 1096-0309 |
| Popis: | We have developed a reliable and rapid immunoassay based on a facile synthesis of fluorescent nanoparticles integrated in immunochromatography technique to quantitatively detect C-reactive protein (CRP). The method is based on a sandwich immunoassay using the Nile-red doped nanoparticles/CRP monoclonal antibody conjugate. The method is simple and fast, with a detection limit of 0.091 mg/L. It provides quantitative analysis in the range of 0.1–160 mg/L, which is adequate for detecting CRP of acute inflammatory or cardiovascular disease. This strategy displayed a good reproducibility and stability to straightforwardly analyze the plasma samples without complicated washing steps, thereby reducing the operating procedures for non-professionals and promoting the detection efficiency and the whole detection process can be completed in 3 min. This approach for carrying out immunoassays can be applied to the detection of CRP in the point-of-care tests. |
| Databáze: | OpenAIRE |
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