Development of a point-of-care technology for bacterial identification in milk
| Autor: | Evgeni Eltzov, Reuven Afriat, Daniel Chalupowicz |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Technology
Point-of-Care Systems Microorganism Biosensing Techniques 02 engineering and technology medicine.disease_cause 01 natural sciences Horseradish peroxidase Analytical Chemistry chemistry.chemical_compound medicine Animals Escherichia coli Immunoassay Chromatography Bacteria medicine.diagnostic_test biology 010401 analytical chemistry Contamination 021001 nanoscience & nanotechnology biology.organism_classification 0104 chemical sciences Milk chemistry biology.protein Colorimetry 0210 nano-technology Biosensor Nitrocellulose |
| Zdroj: | Talanta. 219:121223 |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2020.121223 |
| Popis: | Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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