A Combination of a Polycaprolactone Fumarate Scaffold with Polyethylene Terephthalate Sutures for Intra-Articular Ligament Regeneration
| Autor: | Michael J. Yaszemski, Peter L Kok, Eric R. Wagner, Joshua A Parry, Andre J. van Wijnen, Sanjeev Kakar, Mahrokh Dadsetan |
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| Rok vydání: | 2018 |
| Předmět: |
Scaffold
medicine.medical_specialty Anterior cruciate ligament reconstruction Cell Survival Polyesters medicine.medical_treatment Anterior cruciate ligament Biomedical Engineering Bioengineering Biochemistry Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Suture (anatomy) medicine Polyethylene terephthalate Humans Anterior Cruciate Ligament Cells Cultured Cell Proliferation 030222 orthopedics Anterior Cruciate Ligament Reconstruction Tissue Scaffolds Polyethylene Terephthalates Regeneration (biology) Mesenchymal stem cell Mesenchymal Stem Cells 030229 sport sciences musculoskeletal system Surgery medicine.anatomical_structure chemistry Ligament Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 24:245-253 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2016.0531 |
| Popis: | Intra-articular ligamentous injuries are typically unrepairable and have limited outcomes after graft reconstruction. A combination of porous polycaprolactone fumarate (PCLF) scaffolds with polyethylene terephthalate (PET) sutures was developed with the goal of regenerating intra-articular ligaments. Scaffolds were fabricated by injecting PCLF over three-dimensional-printed molds containing two strands of PET suture down its central pore followed by cross-linking. Scaffolds were seeded with human mesenchymal stem cells (MSCs) from adipose tissue. To demonstrate cell attachment and proliferation in culture, we performed live/dead staining and cell proliferation assays. These experiments showed that MSCs remain viable and continue to proliferate on the scaffolds in culture for at least 2 weeks. Bare scaffolds were then used to reconstruct the rabbit anterior-cruciate ligament (ACL), while control rabbits underwent semitendinosus autograft reconstruction. The specimens underwent micro-computed tomography (CT) imaging, histological examination, and biomechanical testing at 8 weeks. The ultimate pull-out strength of the PCLF-PET scaffolds and tendon autografts was initially 72 ± 30 N and to 45 ± 10 N, respectively (p 0.06). On inspection after 8 weeks in vivo, the intra-articular portion of the PCLF-PET scaffolds was fragmented while the tendon autografts remained intact. Cross-sectional areas of bone tunnels in the PCLF-PET scaffolds (11.3 ± 1 mm |
| Databáze: | OpenAIRE |
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