Reduced expansion of CD94/NKG2C + NK cells in chronic lymphocytic leukemia and CLL-like monoclonal B-cell lymphocytosis is not related to increased human cytomegalovirus seronegativity or NKG2C deletions
| Autor: | Lara Nonell, Anna Puiggros, Xavier Calvo, Miguel López-Botet, Pau Abrisqueta, Ana Ferrer, María Rodríguez-Rivera, Eva Gimeno, Aura Muntasell, Eulàlia Puigdecanet, Mireia Altadill, Blanca Espinet, Gonzalo Blanco, Francesc Bosch, José Yélamos, Magdalena Arnal, Eugenia Abella |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Human cytomegalovirus
Lymphocytosis Monoclonal B cell lymphocytosis HLA-E Chronic lymphocytic leukemia Clinical Biochemistry Population Human leukocyte antigen NKG2C immune system diseases hemic and lymphatic diseases medicine education education.field_of_study business.industry Biochemistry (medical) Hematology General Medicine medicine.disease Monoclonal Immunology Monoclonal B-cell lymphocytosis medicine.symptom business |
| Popis: | Introduction: Dysregulated NK cell-mediated immune responses contribute to tumor evasion in chronic lymphocytic leukemia (CLL), although the NK cell compartment in CLL-like monoclonal B-cell lymphocytosis (MBL) is poorly understood. In healthy individuals, human cytomegalovirus (HCMV) induces the expansion of NK cells expressing high levels of CD94/NKG2C NK cell receptor (NKR) specific for HLA-E. Methods: We analyzed the expression of NKG2A, NKG2C, ILT2, KIR, CD161, and CD57 in 24 MBL and 37 CLL. NKG2C was genotyped in these patients and in 81 additional MBL/CLL, while NKG2C gene expression was assessed in 26 cases. In 8 CLL patients with increased lymphocytosis (≥20 × 109 /L), tumor HLA-E and HLA-G expression was evaluated. Results: NKR distribution did not significantly differ between MBL and CLL patients, although they exhibited reduced NKG2C+ NK cells compared with a non-CLL group (4.6% vs 12.2%, P = .012). HCMV+ patients showed increased percentages of NKG2C+ NK cells compared with HCMV- (7.3% vs 2.9%, P = .176). Frequencies of NKG2C deletions in MBL/CLL were similar to those of the general population. Low/undetectable NKG2C expression was found among NKG2C+/- (45%) and NKG2C+/+ (12%) patients. CLL cases with increased lymphocytosis displayed especially reduced NKG2C expression (1.8% vs 8.1%, P = .029) and tumor cells with high HLA-E (>98%) and variable HLA-G expression (12.4%, range: 0.5-56.4). CLL patients with low NKG2C expression ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|