Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas
| Autor: | Liang-Hui You, Ning Wang, Wei De, Ke-Ming Wang, Li Yuan, Qing-Xin Yuan, Dandan Yin, Xiao-Di Liang, Lin-Tao Wang |
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| Rok vydání: | 2014 |
| Předmět: |
Research Report
medicine.medical_specialty Time Factors endocrine system diseases Cell Apoptosis GPI-Linked Proteins Transfection Rats Sprague-Dawley Islets of Langerhans Downregulation and upregulation In vivo Pregnancy Internal medicine Cell Line Tumor Insulin-Secreting Cells Medicine Animals Mesothelin Cell Proliferation biology business.industry Cell growth Gastroenterology General Medicine Proliferating cell nuclear antigen medicine.anatomical_structure Endocrinology Animals Newborn Gene Expression Regulation biology.protein Cancer research Female RNA Interference business Pancreas Signal Transduction |
| Zdroj: | World journal of gastroenterology. 20(22) |
| ISSN: | 2219-2840 |
| Popis: | AIM: To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats. METHODS: Overexpression or downregulation of mesothelin expression in INS-1 cells was carried out to investigate the effect of mesothelin during cell proliferation and cell apoptosis in vitro. Adenovirus-mediated RNA interference was performed to block mesothelin in vivo to directly assess the role of mesothelin in the remodeling of the endocrine pancreas in neonatal rats. RESULTS: Exogenous overexpression of mesothelin promoted cell proliferation, cell colony formation and enhanced cell resistance to apoptosis of INS-1 cells. Down-regulation of mesothelin made no difference in cell proliferation and apoptosis compared with that in the control group. After an injection of adenovirus-mesothelin, a significantly increased number of small islets appeared, and the expression of PCNA was decreased on day 7 and day 14 compared with the Ad-EGFP group. CONCLUSION: Mesothelin was able to promote β cell proliferation in the remodeling stage of neonatal rats. Mesothelin may have an important role in the remodeling of the endocrine pancreas in neonatal rats. |
| Databáze: | OpenAIRE |
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