Participation of cytoplasmic organelles in E-rosette formation
| Autor: | Tatsuo Suzuta, Sanae A. Ishijima, Hideki Asakura |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
0301 basic medicine
Azides Erythrocytes Rosette Formation T-Lymphocytes Immunology In Vitro Techniques Cycloheximide Biology Microfilament 03 medical and health sciences chemistry.chemical_compound Adenosine Triphosphate 0302 clinical medicine Microtubule Animals Humans Immunology and Allergy Potassium Cyanide Sodium Azide Cytochalasin B Calcimycin Cytochalasin D Organelles Sheep Endoplasmic reticulum Cell Biology Cytochalasins Actin Cytoskeleton Cytosol 030104 developmental biology chemistry Cytoplasm Biophysics 030215 immunology |
| Zdroj: | Immunology and Cell Biology. 69:403-409 |
| ISSN: | 0818-9641 |
| Popis: | To elucidate the mechanism of E-rosette formation between T cells and sheep red blood cells (SRBC), the effect of various agents affecting the function of cytoplasmic structures (microtubules and microfilaments) and organelles (mitochondria and rough endoplasmic reticulum) was investigated. E-rosette formation was greatly inhibited by agents that block either the energy producing system (KCN and NaN3) or the integration of microfilaments (cytochalasin B, dihydrocytochalasin B and cytochalasin D). On the other hand, there was little or no suppression by either inhibitors of protein synthesis (cycloheximide and puromycin), agents that block the polymerization of microtubules (colchicine, podophyllotoxin and vinblastine), or chemicals disconnecting surface membrane proteins from the intracellular structural proteins (chlorpromazine, dibucaine, hydrocortisone, and propranolol). The calcium ionophore A23187, which transports Ca2+ into the cytosol, inhibited the E-rosette formation in the presence of Ca2+, but not in its absence. From these results, we concluded that new synthesis of ATP and the structural integration of microfilaments are indispensable for the E-rosette formation, which is triggered by an interaction between the ligand (T11TS) and its corresponding receptor (CD2). A certain level of intracellular Ca2+ is also involved in the E-rosette formation. |
| Databáze: | OpenAIRE |
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