Lipopolysaccharide-induced Lung Injury in Mice. II. Evaluation of Functional Damage in Isolated Parenchyma Strips
| Autor: | Riccardo Bertini, Gianfranco Caselli, A. Intilangelo, Laura Brandolini, Pellegrini Luigi, R. Chiusaroli, Viviana Ruggieri, C. Asti |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Lipopolysaccharides
Lung Diseases Pulmonary and Respiratory Medicine Serotonin Pathology medicine.medical_specialty Lipopolysaccharide Anti-Inflammatory Agents Bradykinin Substance P Pharmacology Lung injury Betamethasone Proinflammatory cytokine Contractility Mice chemistry.chemical_compound Parenchyma Animals Medicine Drug Interactions Pharmacology (medical) Enzyme Inhibitors Pentoxifylline Methacholine Chloride Dose-Response Relationship Drug business.industry Biochemistry (medical) respiratory system chemistry Female business Histamine Ex vivo Muscle Contraction |
| Zdroj: | Pulmonary Pharmacology & Therapeutics. 13:71-78 |
| ISSN: | 1094-5539 |
| DOI: | 10.1006/pupt.2000.0232 |
| Popis: | Pulmonary inflammatory diseases are characterized by changes in airway responsiveness. This phenomenon is commonly related to the action of inflammatory mediators produced by infiltrated leukocytes. The aim of this study was to investigate in an ex vivo experimental model the effect of acute instillation of lipopolysaccharide (bacterial endotoxin; LPS) on lung parenchyma contractility. We firstly characterized the responsiveness of isolated murine lung to airway stimuli. Murine parenchymal strips were found to be mainly sensitive to 5-hydroxytryptamine (5-HT) while the cholinergic agonist, methacholine (MCh), evoked a smaller contractile response. 5-HT responsiveness was inhibited by methysergide. No significant parenchymal contraction was evoked by histamine, substance P and bradykinin. Lung responsiveness to 5-HT was significantly reduced by in vivo LPS treatment and this effect was only partially paralleled by leukocyte infiltration. In addition, LPS-induced hyporesponsiveness was significantly inhibited by betamethasone (BMS) or pentoxifylline (PTX) pretreatment suggesting that 5-HT lung hyporesponsiveness could be mediated by LPS-induced inflammatory mediators such as inflammatory cytokines. |
| Databáze: | OpenAIRE |
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