Profiling of Selected Functional Metabolites in the Central Nervous System of Marine Medaka (Oryzias melastigma) for Environmental Neurotoxicological Assessments
Autor: | Elva N. Y. Lei, Michael H.W. Lam, Man Shan Yau, Chi Chung Yeung, Margaret B. Murphy, Ka-Leung Wong |
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Rok vydání: | 2016 |
Předmět: |
Central Nervous System
0301 basic medicine Arginine Health Toxicology and Mutagenesis Central nervous system Oryzias 010501 environmental sciences Biology Toxicology 01 natural sciences Serine 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry Valine Toxicity Tests Halogenated Diphenyl Ethers medicine Animals Flame Retardants 0105 earth and related environmental sciences Dansyl Compounds Neurotransmitter Agents General Medicine Metabolism Pollution 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry Leucine Water Pollutants Chemical Chromatography Liquid Toxicant Cysteine |
Zdroj: | Archives of Environmental Contamination and Toxicology. 72:269-280 |
ISSN: | 1432-0703 0090-4341 |
Popis: | The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC-MS/MS quantification. This technique was applied to examine effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days. Multivariate statistical analysis has demonstrated that levels of numerous classical neurotransmitters and their metabolites in the CNS of the fish were perturbed even at the early phase of dietary exposure. Subsequent metabolic pathway analysis further implied potential impairment of the arginine and proline metabolism; glycine, serine and threonine metabolism; D-glutamine and D-glutamate metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis, and the cysteine and methionine metabolism in the brain of the test organism. Our results demonstrate that targeted profiling of functional metabolites in the CNS may shed light on how the various neurological pathways of vertebrates, including humans, are affected by toxicant/stress exposure. |
Databáze: | OpenAIRE |
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