Whole body vibration (WBV) following spinal cord injury (SCI) in rats: Timing of intervention
| Autor: | Stoyan Pavlov, Ramona Jansen, Yasemin Behram Kandemir, Umut Ozsoy, Gregor Stein, Eckhard Schoenau, Klaus Nohroudi, Marilena Manthou, Habib Bendella, Diana S.Y. Abdulla, Doychin N. Angelov, Levent Sarikcioglu, Carolin Meyer, Oliver Semler, Ozlem Ozsoy, Sarah A. Dunlop |
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| Rok vydání: | 2017 |
| Předmět: |
030506 rehabilitation
Statistics as Topic Synaptophysin Hindlimb Severity of Illness Index Vibration 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Glial Fibrillary Acidic Protein medicine Animals Whole body vibration Spasticity Rats Wistar Spinal cord injury Physical Therapy Modalities Spinal Cord Injuries Fixation (histology) Analysis of Variance biology business.industry Calcium-Binding Proteins Microfilament Proteins Recovery of Function medicine.disease Rats Astrogliosis Disease Models Animal Neurology Anesthesia biology.protein Female Neurology (clinical) medicine.symptom 0305 other medical science business Perfusion Locomotion 030217 neurology & neurosurgery |
| Zdroj: | Restorative Neurology and Neuroscience. 35:185-216 |
| ISSN: | 1878-3627 0922-6028 |
| Popis: | Background Following spinal cord injury (SCI), exercise training provides a wide range of benefits and promotes activity-dependent synaptic plasticity. Whole body vibration (WBV) in SCI patients improves walking and spasticity as well as bone and muscle mass. However, little is known about the effects of timing or frequency of intervention. Objective To determine which WBV-onset improves locomotor and bladder functions and influences synaptic plasticity beneficially. Methods SCI was followed by WBV starting 1, 7, 14, 28 days after injury (WBV1, WBV7, etc.) and continued for 12 weeks. Intact animals and those receiving SCI but no WBV (No WBV), SCI plus WBV twice daily (2×WBV) and SCI followed by passive hindlimb flexion-extension (PFE) served as controls. Locomotor [BBB rating, foot stepping angle (FSA) and rump-height index (RHI)] as well as bladder function were determined at 1, 3, 6, 9, and 12 weeks. Following perfusion fixation at 12 weeks, lesion volume and immunofluorescence for astrogliosis (GFAP), microglia (IBA1) and synaptic vesicles (synaptophysin, SYN) were determined. Results Compared to the No WBV group, the WB7 and WBV14 groups showed significantly faster speeds of BBB score recovery though this effect was temporary. Considering RHI we detected a sustained improvement in the WBV14 and PFE groups. Bladder function was better in the WBV14, WBV28, 2×WBV and PFE groups. Synaptophysin levels improved in response to WBV7 and WBV14, but worsened after WBV28 in parallel to an increased IBA1 expression. Correlation- and principal components analysis revealed complex relationships between behavioural (BBB, FSA, RHI) and morphological (GFAP, IBA1, SYN) measurements. Conclusions WBV started 14 days after SCI provides the most benefit (RHI, bladder); starting at 1day after SCI provides no benefit and starting at 28 days may be detrimental. Increasing the intensity of WBV to twice daily did not provide additional benefit. |
| Databáze: | OpenAIRE |
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