Molecular taxonomy using single-strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes
| Autor: | D. F. West, Michael F. Antolin, Christopher H. Dietrich, William C. Black, Joseph Piesman, Douglas E. Norris, S. Kambhampati, R. F. Whitcomb, R. H. D. E. Hiss, Christopher F. Bosio |
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| Rok vydání: | 1994 |
| Předmět: |
Male
Mitochondrial DNA Insecta Wasps Biology DNA Mitochondrial DNA Ribosomal law.invention Hemiptera chemistry.chemical_compound Ticks Species Specificity Aedes law RNA Ribosomal 16S Genetics Animals Molecular Biology Gene Ribosomal DNA Polymorphism Single-Stranded Conformational Polymerase chain reaction Single-strand conformation polymorphism Classification 16S ribosomal RNA Molecular biology chemistry RNA Ribosomal DNA Contamination Aphids Insect Science Female DNA |
| Zdroj: | Insect Molecular Biology. 3:171-182 |
| ISSN: | 1365-2583 0962-1075 |
| DOI: | 10.1111/j.1365-2583.1994.tb00164.x |
| Popis: | Single-strand conformation polymorphism (SSCP) analysis detects single point mutations in DNA molecules. We demonstrate that SSCP analysis of mitochondrial ribosomal DNA (rDNA) genes is a sensitive taxonomic tool because these genes often differ at numerous sites among closely related species. Using conserved primers, portions of the 12S or 16S rDNA genes were amplified using the polymerase chain reaction (PCR) in congeneric species of ticks, leaf hoppers, mosquitoes, and closely related endoparasitic wasps. SSCP was performed and products were visualized with silver staining. Species-specific patterns were observed in all taxa. Intraspecific variation at the level of single nucleotide substitutions was detected. SSCP diagnostics are less expensive and time consuming to develop than PCR with species-specific primers, and, unlike PCR with arbitrary primers, there is minimal concern with DNA contamination from non-target organisms. |
| Databáze: | OpenAIRE |
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