Kinetic Effects on Signal Normalization in Oligonucleotide Microchips with Labeled Immobilized Probes
| Autor: | O. G. Somova, Alexander V. Chudinov, R. A. Yurasov, O. V. Moiseeva, Dmitry Prokopenko, Olga V. Antonova, Dmitry A. Gryadunov, S. V. Pan'kov, V. R. Chechetkin |
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| Rok vydání: | 2009 |
| Předmět: |
DNA
Bacterial Normalization (statistics) endocrine system Chromatography Chemistry Oligonucleotide Gene Expression Profiling Analytical chemistry Mycobacterium tuberculosis General Medicine Carbocyanines Isoniazid resistance Structural Biology Molecular Biology Fluorescent Dyes Oligonucleotide Array Sequence Analysis |
| Zdroj: | Journal of Biomolecular Structure and Dynamics. 27:235-244 |
| ISSN: | 1538-0254 0739-1102 |
| Popis: | Among various factors affecting operation of oligonucleotide microchips, the variations in concentration and in homogeneous distribution of immobilized probes over the cells are one of the most important. The labeling of immobilized probes ensures the complete current monitoring on the probe distribution and is reliable and convenient. Using hydrogel-based oligonucleotide microchips, the applicability of Cy3-labeled immobilized probes for quality control and signal normalization after hybridization with Cy5-labeled target DNA was investigated. This study showed that proper signal normalization should be different in thermodynamic conditions and in transient regime with hybridization far from saturation. This kinetic effect holds for both hydrogel-based and surface oligonucleotide microchips. Besides proving basic features, the technique was assessed on a sampling batch of 50 microchips developed for identifying mutations responsible for rifampicin and isoniazid resistance of Mycobacterium tubercu... |
| Databáze: | OpenAIRE |
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