Oxidative stress in early cystic fibrosis lung disease is exacerbated by airway glutathione deficiency
| Autor: | Arest Cf, Rufus Turner, Peter D. Sly, Luke J. Berry, Nina Dickerhof, Anthony J. Kettle, John F. Pearson, Teagan S. Hoskin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Antioxidant Cystic Fibrosis Neutrophils medicine.medical_treatment medicine.disease_cause Biochemistry Cystic fibrosis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) medicine Humans Sulfones Age of Onset Allantoin Child Lung Peroxidase Inflammation biology medicine.diagnostic_test Glutathione Disulfide Infant Glutathione medicine.disease Bronchiectasis Oxidative Stress 030104 developmental biology Bronchoalveolar lavage 030228 respiratory system chemistry Myeloperoxidase Case-Control Studies Child Preschool Immunology biology.protein Glutathione disulfide Female Bronchoalveolar Lavage Fluid Oxidation-Reduction Oxidative stress |
| Zdroj: | Free radical biologymedicine. 113 |
| ISSN: | 1873-4596 |
| Popis: | Neutrophil-derived myeloperoxidase (MPO) is recognized as a major source of oxidative stress at the airway surface of a cystic fibrosis (CF) lung where, despite limited evidence, the antioxidant glutathione is widely considered to be low. The aims of this study were to establish whether oxidative stress or glutathione status are associated with bronchiectasis and whether glutathione deficiency is inherently linked to CF or a consequence of oxidative stress. MPO was measured by ELISA in 577 bronchoalveolar lavage samples from 205 clinically-phenotyped infants and children with CF and 58 children without CF (ages 0.2-6.92 years). Reduced glutathione (GSH), oxidized glutathione species (GSSG; glutathione attached to proteins, GSSP; glutathione sulfonamide, GSA) and allantoin, an oxidation product of uric acid, were measured by mass spectrometry. The odds of having bronchiectasis were associated with MPO and GSSP. GSH was low in children with CF irrespective of oxidation. Oxidized glutathione species were significantly elevated in CF children with pulmonary infections compared to uninfected CF children. In non-CF children, infections had no effect on glutathione levels. An inadequate antioxidant response to neutrophil-mediated oxidative stress during infections exists in CF due to an inherent glutathione deficiency. Effective delivery of glutathione and inhibition of MPO may slow the development of bronchiectasis. |
| Databáze: | OpenAIRE |
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