Molecular identification of triticale introgression lines carrying leaf rust resistance genes transferred from Aegilops kotschyi Boiss. and Ae. tauschii Coss
| Autor: | Aleksandra Noweiska, Jolanta Belter, Roksana Skowrońska, Michał Kwiatek, Halina Wiśniewska, Waldemar Ulaszewski |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Secale Aegilops Introgression Chromosomal translocation Resistance genes Biology Genes Plant 01 natural sciences Rust Chromosomes Plant 03 medical and health sciences Plant Genetics • Original Paper Genetics Aegilops kotschyi Genetic variability Disease Resistance Plant Diseases Genomic in situ hybridization. Leaf rust Basidiomycota fungi food and beverages Molecular markers General Medicine Triticale biology.organism_classification Horticulture 030104 developmental biology 010606 plant biology & botany |
| Zdroj: | Journal of Applied Genetics |
| ISSN: | 2190-3883 1234-1983 |
| Popis: | Triticale (×Triticosecale Wittmack) is a commercial hybrid harboring wheat (Triticum sp.) and rye (Secale cereale L.) genomes. The limited genetic diversity of this crop resulted in the collapse of fungal disease resistance. Leaf rust disease, caused by Puccinia triticina Eriks. is reported to reduce the triticale yield significantly (more than 30%). There is a need to enlarge the genetic variability of this crop including leaf resistance genes. The main aim of this research was to transfer Lr39 and Lr54 leaf rust resistance genes into triticale from Aegilops tauschii and Ae. kotschyi, respectively. A reaction of seedlings of 200 plants of two triticale-Aegilops translocation lines (Bogo-2Dt.2R and Sekundo-2Sk.2R) was compared after inoculation with a natural mixture of P. triticina races, specific to triticale. Before inoculation, each plant was screened using molecular cytogenetics and molecular markers linked to leaf rust resistance genes. Presence of Aegilops chromosome segments was confirmed using genomic in situ hybridization (GISH). Lr39 and Lr54 leaf rust resistance genes were identified using Xgdm35 and S14 molecular markers, respectively. After inoculation, a significant improvement of resistance severity was observed in Sekundo-2Sk.2R in comparison with triticale cv. Sekundo plants. The resistance level of Bogo-2Dt.2R did not differ compared to triticale cv. Bogo plants. It was shown, that Lr39 gene did not increase the leaf rust resistance level of triticale cv. Bogo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink