Probing protein nanopores with poly(ethylene glycol)s

Autor:	Wenxing Liu, Ekaterina M. Nestorovich
Rok vydání:	2022
Předmět:	Nanopores Polymers Proteins Molecular Biology Biochemistry Polyethylene Glycols
Zdroj:	PROTEOMICS. 22:2100055
ISSN:	1615-9861 1615-9853
DOI:	10.1002/pmic.202100055
Popis:	Neutral water-soluble poly(ethylene glycol)s (PEGs) have been extensively explored in protein nanopore research for the past several decades. The principal use of PEGs is to investigate the membrane protein ion channel physical characteristics and transport properties. In addition, protein nanopores are used to study polymer-protein interactions and polymer physicochemical properties. In this review, we focus on the biophysical studies on probing protein ion channels with PEGs, specifically on nanopore sizing by PEG partitioning. We discuss the fluctuation analysis of ion channel currents in response to the PEGs moving within their confined geometries. The advantages, limitations, and recent developments of the approach are also addressed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::629f535f024450e53026a67778f58bf5 https://doi.org/10.1002/pmic.202100055 Zobrazit plný text záznamu Plný text