Reproductive biology of the native forage grass Trichloris crinita (Poaceae, Chloridoideae)
| Autor: | Florencia Galdeano, Camilo Luis Quarin, Pablo Federico Cavagnaro, Karina Barboza, Perla Carolina Kozub, Juan Bruno Cavagnaro |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Progeny testing Heterozygote food.ingredient Pollination Trichloris crinita Outcrossing Flowers Self-Fertilization Plant Science Poaceae 010603 evolutionary biology 01 natural sciences food Botany Animals Inbreeding Ecology Evolution Behavior and Systematics biology General Medicine Flow Cytometry biology.organism_classification Sexual reproduction Seeds Chloridoideae Trichloris Microsatellite Repeats 010606 plant biology & botany |
| Zdroj: | Plant Biology. 19:444-453 |
| ISSN: | 1435-8603 |
| Popis: | Trichloris crinita is a perennial forage grass species native to arid regions of the American continent. Due to its extensive area of distribution, good forage quality, and resistance to drought and grazing, this species is widely utilized as forage and for revegetation purposes in environments with low water availability. Despite its importance, genetic improvement of T. crinita has been very limited, partly as consequence of the lack of knowledge on its mode of reproduction. In the present work, we studied the reproductive biology of T. crinita by means of embryological analyses, flow cytometric seed screen (FCSS), self-compatibility tests and progeny testing with morphological and molecular markers. Cytological analyses revealed embryo sacs with eight nuclei and of Polygonum type for all T. crinita accessions analyzed. FCSS histograms exhibited two clear peaks corresponding to 2C and 3C DNA contents, indicating embryo sacs of sexual origin. Controlled pollination experiments designed to evaluate seed set (%) demonstrated that T. crinita is self-compatible, whereas results from morphological and simple sequence repeat (SSR) marker analysis of progenies revealed lack of outcrossing. Altogether, these results indicate that T. crinita reproduces sexually. It is a self-compatible and autogamous species. It is expected that these data will have a positive impact in the genetics and breeding of this species, and therefore contribute to its proper utilization in arid regions. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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