2-LTR Circular Viral DNA as a Marker for Human Immunodeficiency Virus Type 1 Infection in Vivo
| Autor: | C. D. Pauza, F. M. Graziano, Parul Trivedi, T. S. Mckechnie, D. D. Richman |
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| Rok vydání: | 1994 |
| Předmět: |
viruses
Molecular Sequence Data HIV Infections Biology Peripheral blood mononuclear cell Zalcitabine chemistry.chemical_compound Zidovudine Virology medicine Humans Didanosine HIV Long Terminal Repeat Base Sequence Long terminal repeat CD4 Lymphocyte Count chemistry DNA Viral HIV-1 Leukocytes Mononuclear DNA Circular Viral load Biomarkers DNA Half-Life medicine.drug |
| Zdroj: | Virology. 205:470-478 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1994.1667 |
| Popis: | The unintegrated viral DNA found in human immunodeficiency virus type 1 infection includes linear and circular forms. We targeted the circular form containing two copies of the viral long terminal repeat (2-LTR circle) and developed specific assays to detect this molecule in peripheral blood mononuclear cells from HIV-infected patients. In vitro HIV-1 infection of peripheral blood mononuclear cells showed rapid accumulation and rapid decay of 2-LTR circular viral DNA. Examination of 2-LTR circular viral DNA levels provides a view of spreading infection based on a viral DNA form that is structurally distinct and has a known, short half-life in infected cells. In patients not receiving antiviral therapy, the levels of 2-LTR circular viral DNA and total viral DNA were significantly correlated to CD4 cell counts. Similar correlations were not observed in patients receiving zidovudine (AZT), didanosine (ddA), or zalcitabine (ddC). |
| Databáze: | OpenAIRE |
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