A cell-free ROS-responsive hydrogel/oriented poly(lactide-co-glycolide) hybrid scaffold for reducing inflammation and restoring full-thickness cartilage defects
| Autor: | Wangbei Cao, Xinyu Wu, Changyou Gao, Liyin Shen, Xue Feng, Zhongru Gou, Chenxi Tu, Zhaoyi Wang, Tong Zhou, Jieqi Xie, Liwen Deng, Jie Ding, Peifang Xu, Yang Zhu |
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| Rok vydání: | 2021 |
| Předmět: |
Cartilage
Articular Male Scaffold Cell Survival Biomedical Engineering Bioengineering Biocompatible Materials Biomaterials Glycosaminoglycan chemistry.chemical_compound In vivo Cell Line Tumor Hyaluronic acid medicine Animals Humans Polyglactin 910 Inflammation Tissue Scaffolds Chemistry Hyaline cartilage Cartilage Regeneration (biology) technology industry and agriculture Hydrogels PLGA medicine.anatomical_structure Biophysics Rabbits Reactive Oxygen Species |
| Zdroj: | Biomedical materials (Bristol, England). 16(6) |
| ISSN: | 1748-605X |
| Popis: | The modulation of inflammation in tissue microenvironment takes an important role in cartilage repair and regeneration. In this study, a novel hybrid scaffold was designed and fabricated by filling a reactive oxygen species (ROS)-scavenging hydrogel (RS Gel) into a radially oriented poly(lactide-co-glycolide) (PLGA) scaffold. The radially oriented PLGA scaffolds were fabricated through a temperature gradient-guided phase separation and freeze-drying method. The RS Gel was formed by crosslinking the mixture of ROS-responsive hyperbranched polymers and biocompatible methacrylated hyaluronic acid (HA-MA). The hybrid scaffolds exhibited a proper compressive modulus, good ROS-scavenging capability, and cell compatibility.In vivotests showed that the hybrid scaffolds significantly regulated inflammation and promoted regeneration of hyaline cartilage after they were implanted into full-thickness cartilage defects in rabbits for 12 w. In comparison with the PLGA scaffolds, the neo-cartilage in the hybrid scaffolds group possessed more deposition of glycosaminoglycans and collagen type II, and were well integrated with the surrounding tissue. |
| Databáze: | OpenAIRE |
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