Genetic and biological characterisation of Zika virus isolates from different Brazilian regions
| Autor: | Diogo Kuczera, Helisson Faoro, Allan Henrique Depieri Cataneo, Camila Zanluca, Juliano Bordignon, Nathalia Cavalheiro Auwerter, Pryscilla Fanini Wowk, Daisy Maria Strottmann, Ana Luiza Pamplona Mosimann, Claudia Nunes Duarte dos Santos, Andrea Cristine Koishi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
Nonsynonymous substitution lcsh:Arctic medicine. Tropical medicine Virus Cultivation molecular markers lcsh:RC955-962 030231 tropical medicine Population lcsh:QR1-502 biological characterisation Biology Virus Replication lcsh:Microbiology Zika virus Mice 03 medical and health sciences 0302 clinical medicine Aedes Chlorocebus aethiops Animals Humans education Vero Cells Phylogeny Infectivity Mice Inbred BALB C education.field_of_study Zika Virus Infection Flavivirus Zika Virus Viral Load biology.organism_classification Virology Viral replication Vero cell Original Article Viral load Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz Memórias do Instituto Oswaldo Cruz., Vol 114 |
| ISSN: | 1678-8060 0074-0276 |
| DOI: | 10.1590/0074-02760190150 |
| Popis: | BACKGROUND Zika virus (ZIKV) infections reported in recent epidemics have been linked to clinical complications that had never been associated with ZIKV before. Adaptive mutations could have contributed to the successful emergence of ZIKV as a global health threat to a nonimmune population. However, the causal relationships between the ZIKV genetic determinants, the pathogenesis and the rapid spread in Latin America and in the Caribbean remain widely unknown. OBJECTIVES The aim of this study was to characterise three ZIKV isolates obtained from patient samples during the 2015/2016 Brazilian epidemics. METHODS The ZIKV genomes of these strains were completely sequenced and in vitro infection kinetics experiments were carried out in cell lines and human primary cells. FINDINGS Eight nonsynonymous substitutions throughout the viral genome of the three Brazilian isolates were identified. Infection kinetics experiments were carried out with mammalian cell lines A549, Huh7.5, Vero E6 and human monocyte-derived dendritic cells (mdDCs) and insect cells (Aag2, C6/36 and AP61) and suggest that some of these mutations might be associated with distinct viral fitness. The clinical isolates also presented differences in their infectivity rates when compared to the well-established ZIKV strains (MR766 and PE243), especially in their abilities to infect mammalian cells. MAIN CONCLUSIONS Genomic analysis of three recent ZIKV isolates revealed some nonsynonymous substitutions, which could have an impact on the viral fitness in mammalian and insect cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|