Capillary electrophoresis-mass spectrometry at trial by metabo-ring
| Autor: | Marianne Fillet, Cyril Colas, N. Thuy Tran, Erika P. Portero, Meera Shanmuganathan, Jan Nouta, Marton Szigeti, Christopher Lößner, Elena Tobolkina, Herbert Lindner, Peter Nemes, Jens Meixner, Iseult Lynch, J.A. Thorn, Marie-Jia Gou, Nicolas Drouin, Sabrina Ferré, Christian Neusüß, Guinevere S. M. Lageveen-Kammeijer, Zachary Kroezen, Rouba Nasreddine, Ghassan Al Hamoui Dit Banni, Santiago Codesido, Myriam Taverna, András Guttman, Klaus Faserl, Marlien van Mever, Ángeles López Gonzálvez, Wei Zhang, Rawi Ramautar, Claudia Bich-Muracciole, Antony Lechner, Manuel Nelke, Serge Rudaz, Reine Nehmé, Laurent Nyssen, Víctor González-Ruiz, Andrew J. Chetwynd, Stefan Lämmerer, Anne-Catherine Servais, Sylvie Liu, Coral Barbas, Thomas Hankemeier, Catherine Perrin, Yannis François, Philip Britz-McKibbin |
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| Přispěvatelé: | Chimie de la matière complexe (CMC), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biomarker identification
Computational chemistry Metabolite Interfaces 010402 general chemistry Mass spectrometry 01 natural sciences Capillary electrophoresis–mass spectrometry Article Analytical Chemistry chemistry.chemical_compound Electrolytes Capillary electrophoresis Metabolomics Tandem Mass Spectrometry [CHIM.ANAL]Chemical Sciences/Analytical chemistry Cations Genetics Humans Organic Chemicals Reproducibility ddc:615 Chromatography Chemistry 010401 analytical chemistry Electrophoresis Capillary Reproducibility of Results 0104 chemical sciences Electrophoresis Metabolism Metabolome Databases Chemical |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2020, 92 (20), pp.14103-14112. ⟨10.1021/acs.analchem.0c03129⟩ Analytical Chemistry, Vol. 92, No 20 (2020) pp. 14103-14112 Analytical Chemistry, 92(20), 14103-14112. AMER CHEMICAL SOC |
| ISSN: | 0003-2700 1520-6882 |
| Popis: | Capillary zone electrophoresis-mass spectrometry (CE-MS) is a mature analytical tool for the efficient profiling of (highly) polar and ionizable compounds. However, the use of CE-MS in comparison to other separation techniques remains underrepresented in metabolomics, as this analytical approach is still perceived as technically challenging and less reproducible, notably for migration time. The latter is key for a reliable comparison of metabolic profiles and for unknown biomarker identification that is complementary to high resolution MS/MS. In this work, we present the results of a Metabo-ring trial involving 16 CE-MS platforms among 13 different laboratories spanning two continents. The goal was to assess the reproducibility and identification capability of CE-MS by employing effective electrophoretic mobility (mu(eff)) as the key parameter in comparison to the relative migration time (RMT) approach. For this purpose, a representative cationic metabolite mixture in water, pretreated human plasma, and urine samples spiked with the same metabolite mixture were used and distributed for analysis by all laboratories. The mu(eff) was determined for all metabolites spiked into each sample. The background electrolyte (BGE) was prepared and employed by each participating lab following the same protocol. All other parameters (capillary, interface, injection volume, voltage ramp, temperature, capillary conditioning, and rinsing procedure, etc.) were left to the discretion of the contributing laboratories. The results revealed that the reproducibility of the mu(eff) for 20 out of the 21 model compounds was below 3.1% vs 10.9% for RMT, regardless of the huge heterogeneity in experimental conditions and platforms across the 13 laboratories. Overall, this Metabo-ring trial demonstrated that CE-MS is a viable and reproducible approach for metabolomics. |
| Databáze: | OpenAIRE |
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