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Levitsky A. P., Markov A. V., Velichko V. V., Selivanskaya I. A., Lapinskaya A. P. Effect of an antidisbiotic agent on the biosynthesis of fatty acids of liver lipids of rats which received palm oil on the background of dysbiosis. Journal of Education, Health and Sport. 2022;12(5):292-303. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.05.023 https://apcz.umk.pl/JEHS/article/view/JEHS.2022.12.05.023 https://zenodo.org/record/6607847 The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 1, 2021. No. 32343. Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences). Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 1 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159. Przypisane dyscypliny naukowe:Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu). © The Authors 2022; This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 29.04.2022. Revised: 11.05.2022. Accepted: 31.05.2022. UDC 616.314+604.315 EFFECT OF AN ANTIDISBIOTIC AGENT ON THE BIOSYNTHESIS OF FATTY ACIDS OF LIVER LIPIDS OF RATS WHICH RECEIVED PALM OIL ON THE BACKGROUND OF DYSBIOSIS A. P. Levitsky1, A. V. Markov2, V. V. Velichko3, I. A. Selivanskaya3, A. P. Lapinskaya1 1Odessa National Technology University, Odessa, Ukraine 2Lviv National Medical University named after Danylo Galytskij, Lviv, Ukraine 3Odessa National Medical University, Odessa, Ukraine Abstract Background. To determine the effect of antidysbiotic agent on the biosynthesis of fatty acids of lipids in the liver of rats fed a high-fat diet (HFD) with palm oil against the background of dysbiosis. Methods. The HFD contained 15% palm oil. In a biological experiment, white rats were used, divided into 4 groups: the 1st group received a fat-free diet (FFD), the 2nd, 3rd, and 4th received HFD. In rats of the 3rd and 4th groups, dysbiosis was reproduced using lincomycin. Rats of the 4th group from the first day of the experiment received an antidysbiotic agent (inulin + quercetin, ADA) with food. The duration of feeding is 39 days. Liver lipids were divided into 3 fractions: neutral lipids (NL), phospholipids (PL), and free fatty acids (FFA). The fatty acid composition of the fractions was determined by gas chromatography. The “activities” of fatty acid synthase, palmitic acid elongase, and stearyl-CoA- desaturase (SCD18 and SCD16) were determined. Results. The presence of all classes of fatty acids (FA) in the liver lipids of rats treated with FFD was established. Consumption of HFD with palm oil increased the content of NL in the liver by 6 times (gr. 2), and in group 3 by 8 times. The introduction of ADA reduces the content of NL almost to the level of the 1st group. The content of ω-3 polyunsaturated fatty acids (PUFA) decreases in the PL fraction in rats of the 3rd group and is restored in rats of the 4th group. In rats treated with HFD, the "activity" of synthase, SCD18 and very strongly SCD16 are reduced. Conclusion: The negative effect of palm oil on the background of dysbiosis on the biosynthesis of fatty acids in the liver, leading to hepatic steatosis and deficiency of ω-3 PUFA, can be prevented by the use of an antidysbiotic agent. Keywords: palm oil; fatty acids; liver lipids; dysbiosis; antidysbiotic agent. |
