Multiple heart-cutting and comprehensive two-dimensional liquid chromatography hyphenated to mass spectrometry for the characterization of the antibody-drug conjugate ado-trastuzumab emtansine
| Autor: | Gerd Vanhoenacker, Isabel Vandenheede, Koen Sandra, Maureen Joseph, Pat Sandra, Mieke Steenbeke |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antibody-drug conjugate Immunoconjugates Ado-trastuzumab emtansine Clinical Biochemistry Peptide Antineoplastic Agents Mass spectrometry Tandem mass spectrometry Ado-Trastuzumab Emtansine Antibodies Monoclonal Humanized 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences Drug conjugation Tandem Mass Spectrometry Humans Maytansine Amino Acid Sequence chemistry.chemical_classification Chromatography Chemistry Lysine 010401 analytical chemistry Cell Biology General Medicine Equipment Design Trastuzumab Combinatorial chemistry 0104 chemical sciences Characterization (materials science) 030104 developmental biology Conjugate Chromatography Liquid |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 1032 |
| ISSN: | 1873-376X |
| Popis: | Antibody-drug conjugates might be the magic bullets referred to by Paul Ehrlich over 100 years ago. Together with a huge therapeutic potential, these molecules come with a structural complexity that drives state-of-the-art chromatography and mass spectrometry to its limits. The use of multiple heart-cutting (mLC-LC) and comprehensive (LC×LC) multidimensional LC in combination with high resolution mass spectrometry for the characterization of the lysine conjugated antibody-drug conjugate ado-trastuzumab emtansine, commercialized as Kadcyla, is presented. By combining protein and peptide measurements, attributes such as drug loading, drug distribution and drug conjugation sites can be assessed in an elegant manner. |
| Databáze: | OpenAIRE |
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