Solute Carrier Family 27 Member 4 (SLC27A4) Enhances Cell Growth, Migration, and Invasion in Breast Cancer Cells
Autor: | Jung-Yu Kan, Shih-Kai Chou, Meng-Chi Yen, Ming-Feng Hou, Po-Lin Kuo, Ya-Ling Hsu |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
fatty acid transport protein 4 (FATP4) Vimentin migration lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine Cell Movement lipid metabolism Gene Regulatory Networks skin and connective tissue diseases lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification biology Cell Cycle Fatty Acids General Medicine Cell cycle Fatty Acid Transport Proteins invasion very long-chain acyl-CoA synthetases member 4 (ACSVL4) Computer Science Applications Gene Expression Regulation Neoplastic 030220 oncology & carcinogenesis Female Signal Transduction Epithelial-Mesenchymal Transition solute carrier family 27 member 4 (SLC27A4) proliferation Breast Neoplasms Article Catalysis Inorganic Chemistry 03 medical and health sciences Breast cancer breast cancer Cell Line Tumor fatty acid transporter medicine Humans Neoplasm Invasiveness Gene Silencing Physical and Theoretical Chemistry Molecular Biology Cell Proliferation Fatty acid metabolism Cell growth Organic Chemistry Fatty acid medicine.disease Solute carrier family 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Tumor progression biology.protein Cancer research |
Zdroj: | International Journal of Molecular Sciences Volume 19 Issue 11 International Journal of Molecular Sciences, Vol 19, Iss 11, p 3434 (2018) |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms19113434 |
Popis: | Fatty acid metabolism is important in the regulation of breast cancer progression. Some of the proteins involved in fatty acid transport have been demonstrated to promote the proliferation, migration, and invasion in breast cancer cells. Solute carrier family 27 member 4 (SLC27A4) is a fatty acid transporter protein and is related to very long chain acyl-CoA synthetase activity. In the present study, bioinformatic analysis revealed that relatively high SLC27A4 expression was observed in all subtypes of breast tumor tissues when compared to normal breast tissues. Silencing SLC27A4 expression significantly reduced uptake of free fatty acids in two breast cancer cell lines, Hs578T and MDA-MB-231. Cell growth inhibition was observed in SLC27A4-silenced Hs578T and cell cycle was arrested at G2/M. In addition, the capacity of migration and invasion decreased in both cell lines after knockdown of SLC27A4. The epithelial&ndash mesenchymal transition signaling pathway was inhibited because protein expression of Slug, vimentin, &alpha smooth muscle actin, and other regulators was lower than that in control cells. Taken together, our results confirm that high SLC27A4 is associated with tumor progression in breast cancer cells. It is worth investigating whether SLC27A4 serves a diagnostic marker and therapeutic target in further studies. |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |