Whole-Genome Scans Provide Evidence of Adaptive Evolution in Malawian Plasmodium falciparum Isolates
| Autor: | Kevin Marsh, Abdoulaye A. Djimde, Jacqui Montgomery, Issaka Zongo, Mark D. Preston, Alexis Nzila, Harold Ocholla, Susana Campino, Ogobara K. Doumbo, Alister Craig, Dominic P. Kwiatkowski, Anja T. R. Jensen, Daniel Alcock, Samuel Assefa, Jean Bosco Oudraogo, Steffen Borrmann, Mwapatsa Mipando, Rick M. Fairhurst, François Nosten, Tim J. Anderson, Taane G. Clark, Bronwyn MacInnis, Anja Terlouw |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Malawi Linkage disequilibrium genetic epidemiology Plasmodium falciparum Population Adaptation Biological Population genetics wa_395 Evolution Molecular Major Articles and Brief Reports Antimalarials parasitic diseases Genetic variation medicine Humans Immunology and Allergy Parasites Malaria Falciparum Selection Genetic Child education Allele frequency Genetics Molecular Epidemiology Genetic diversity education.field_of_study biology Genetic Variation Sequence Analysis DNA medicine.disease biology.organism_classification 3. Good health ws_300 Infectious Diseases qx_135 Child Preschool qu_550 Female qu_470 genomes Genome Protozoan Malaria |
| Zdroj: | The Journal of Infectious Diseases Ocholla, H, Preston, M D, Mipando, M, Jensen, A T R, Campino, S, MacInnis, B, Alcock, D, Terlouw, A, Zongo, I, Oudraogo, J-B, Djimde, A A, Assefa, S, Doumbo, O K, Borrmann, S, Nzila, A, Marsh, K, Fairhurst, R M, Nosten, F, Anderson, T J C, Kwiatkowski, D P, Craig, A, Clark, T G & Montgomery, J 2014, ' Whole-Genome Scans Provide Evidence of Adaptive Evolution in Malawian Plasmodium falciparum Isolates ', The Journal of Infectious Diseases, vol. 210, no. 12, pp. 1991-2000 . https://doi.org/10.1093/infdis/jiu349 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/jiu349 |
| Popis: | BACKGROUND\ud Selection by host immunity and antimalarial drugs has driven extensive adaptive evolution in Plasmodium falciparum, and continues to produce ever-changing landscapes of genetic variation.\ud \ud METHODS\ud We carried out whole-genome sequencing of 69 P. falciparum isolates from Malawi and used population genetics approaches to investigate genetic diversity and population structure, and identify loci under selection.\ud \ud RESULTS\ud High genetic diversity (π=2.4 x 10(-4)), moderately high multiplicity of infection (2.7), and low linkage disequilibrium (500-bp) were observed in Chikhwawa District, Malawi, an area of high malaria transmission. Allele frequency-based tests provided evidence of recent population growth in Malawi and detected potential targets of host immunity and candidate vaccine antigens. Comparing the sequence variation between isolates from Malawi and those from 5 geographically dispersed countries (Kenya, Burkina Faso, Mali, Cambodia, and Thailand) detected population genetic differences between Africa and Asia, within Southeast Asia, and within Africa. Applying haplotype-based tests of selection to sequence data from all 6 populations identified signals of directional selection at known drug-resistance loci, including pfcrt, pfdhps, pfmdr1, and pfgch1.\ud \ud CONCLUSIONS\ud The sequence variations observed at drug-resistance loci reflect differences in each country's historical use of antimalarial drugs, and may be useful in formulating local malaria treatment guidelines. |
| Databáze: | OpenAIRE |
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