Dendritic Cells Transmit Human Immunodeficiency Virus Type 1 to Monocytes and Monocyte-Derived Macrophages
| Autor: | Franz Steindl, Manfred P. Dierich, Ines Frank, Brigitte Müllauer, Georg M. Sprinzl, Martin Spruth, Laco Kacani, Michael G. Schwendinger |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
medicine.drug_class
Immunology CD11c CD18 Viremia Monoclonal antibody Microbiology Monocytes Virus Immunoenzyme Techniques Virology Animal Viruses medicine Humans Staining and Labeling biology Cell adhesion molecule Macrophages Monocyte virus diseases Dendritic Cells medicine.disease Coculture Techniques medicine.anatomical_structure Integrin alpha M Insect Science HIV-1 biology.protein |
| Zdroj: | Journal of Virology. 72:6671-6677 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.72.8.6671-6677.1998 |
| Popis: | Previous studies have shown that human immunodeficiency virus type 1 (HIV-1) exploits dendritic cells (DC) to replicate and spread among CD4 + T cells. To explain the predominance of non-syncytium-inducing (NSI) over syncytium-inducing (SI) strains during the initial viremia of HIV, we investigated the ability of blood monocyte (Mo)-derived DC to transmit HIV-1 to CD4 + cells of the monocytoid lineage. First, we demonstrate that in our system, DC are able to transmit NSI strains, but not SI strains, of HIV-1 to fresh blood Mo and to Mo-derived macrophages (MDM). To establish a productive infection, a 10-fold-lower amount of virus was necessary for DC-mediated transmission of HIV-1 to Mo than in case of cell-free infection. Second, immature CD83 − DC (imDC) transmit virus to Mo and MDM with higher efficacy compared to mature CD83 + DC (maDC); this finding is in contrast to data previously obtained with CD4 + T cells. Third, maturation from imDC to maDC efficiently silenced expression of β 2 -integrins CD11b, CD11c, and CD18 by maDC. Moreover, monoclonal antibody against CD18 inhibited transmission of HIV-1 from imDC to Mo. We propose that the adhesion molecules of the CD11/CD18 family, involved in cell-cell interactions of DC with the microenvironment, may play a major role in imDC-mediated HIV-1 infection of Mo and MDM. |
| Databáze: | OpenAIRE |
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