A thermoresistant strain of feline immunodeficiency virus (FIV) with an altered cytopathic effect and a restricted cell tropism
| Autor: | Jacqueline Braunwald, André Kirn, F. Koehren, Jean-Pierre Martin, C. Alix |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Feline immunodeficiency virus
Hot Temperature viruses Molecular Sequence Data Bone Marrow Cells Thymus Gland Biology Immunodeficiency Virus Feline Kidney Virus Replication Genes env Virus Cell Line Viral envelope Cytopathogenic Effect Viral Virology Animals Tropism Cells Cultured Cytopathic effect Syncytium Cell fusion Macrophages General Medicine biology.organism_classification Phenotype Viral replication Cats Leukocytes Mononuclear Lymph Nodes |
| Zdroj: | Archives of virology. 143(11) |
| ISSN: | 0304-8608 |
| Popis: | A thermoresistant strain, designated m41, of feline immunodeficiency virus (FIV) was selected after 31 successive passages of chronically infected IRC4 cells at 41 degrees C. The wild-type virus (wt) which served as a control was cultivated the same number of times at 37 degrees C. In Crandell feline kidney cells (CrFK), the replication of m41 was similar at 37 degrees C and 41 degrees C, whereas wt multiplied only at 37 degrees C. Furthermore, m41 was more resistant than the wt strain at temperatures ranging from 37 to 56 degrees C. Syncytia formation was observed with m41 when the CrFK were incubated at 41 degrees C whereas neither m41 nor wt produced syncytia at 37 degrees C. The level of replication of wt and m41 on feline lymphoid primary cells at 37 degrees C was similar. In contrast to wt, m41 was unable to infect bone marrow macrophages. Since one or several mutations in the envelope (env) gene could be involved in changes of cell fusion properties and of cellular tropism, the nucleotide sequence of the env gene derived from wt and m41 respectively was determined. Ten mutations were found in the env gene of m41, thus leading to 9 amino acid modifications in the envelope glycoproteins. These results suggest that structural modifications of the viral envelope proteins are prerequisites for the replication of a thermoresistant FIV strain at elevated temperature and are correlated with the newly acquired viral phenotype. |
| Databáze: | OpenAIRE |
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