Establishment of an in vitro system representing the chicken gut-associated lymphoid tissue
| Autor: | Ye Wen Kristeen-Teo, Noorjahan Banu Alitheen, Peter McCullagh, Swee Keong Yeap, Sheau Wei Tan, S.J. McClure |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Gut-associated lymphoid tissue
lcsh:Medicine Chick Embryo Epithelium Ornithology Cell Movement Lymphoid Organs Bursa of Fabricius lcsh:Science Cells Cultured Cell Aggregation B-Lymphocytes education.field_of_study Multidisciplinary Fluoresceins Cell aggregation Cell biology Phenotype medicine.anatomical_structure Veterinary Diseases Medicine Research Article Veterinary Medicine animal structures Newcastle Disease Immunology Population Newcastle disease virus Succinimides Spleen Biology Real-Time Polymerase Chain Reaction Veterinary Immunology Fluorescence Cytidine Deaminase medicine Animals education B cell Cell Proliferation Peroxidase Staining and Labeling Cell growth Cell Membrane lcsh:R Epithelial Cells Veterinary Virology Virology Gastrointestinal Tract Ki-67 Antigen Immune System Clinical Immunology Veterinary Science lcsh:Q Zoology Chickens |
| Zdroj: | PLoS ONE, Vol 7, Iss 11, p e49188 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined. |
| Databáze: | OpenAIRE |
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