Effects of CpG oligodeoxynucleotides on the differentiation of Treg/Th17 cells
| Autor: | Aili He, Liufang Gu, Pengyu Zhang, Xiao-Rong Ma, Wan-Hong Zhao, Yueli Wang, Hailing Liu, Wanggang Zhang, Yi-Lin Zhang, Yuqiang Ji, Jin Wang, Bo Lei |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
medicine.drug_class
CpG Oligodeoxynucleotide Cellular differentiation Immunology chemical and pharmacologic phenomena Biology Monoclonal antibody T-Lymphocytes Regulatory Mice RAR-related orphan receptor gamma Transforming Growth Factor beta medicine Animals RNA Messenger Molecular Biology Cells Cultured Interleukin FOXP3 hemic and immune systems Cell Differentiation Forkhead Transcription Factors medicine.disease Mice Inbred C57BL Leukemia CpG site Oligodeoxyribonucleotides Cancer research Cytokines Th17 Cells Female |
| Zdroj: | Molecular immunology. 132 |
| ISSN: | 1872-9142 |
| Popis: | The balance between Th17 cells and T regulatory (Treg) cells has emerged as a prominent factor in regulating cancer development. However, the effect of CpG oligodeoxynucleotides (ODNs) on the differentiation of Treg/Th17 cells has not been well studied. We sought here to explore the function of CpG ODNs in the differentiation of Tregs and Th17 cells in vitro and in vivo.Mouse spleen cells were cultured with anti-CD3 monoclonal antibodies in vitro. Tregs and Th17 cell differentiation was induced by transforming growth factor (TGF)-β and interleukin (IL)-2, or TGF-β, IL-6, and IL-23, respectively. Then cells were treated with two CpG ODNs, CpG 1982, or CpG 1826. FBL-3-inoculated C57Bl/6 mice were treated with CpG 1826, tumor vaccine, or combination of CpG 1826 and tumor vaccine. After treatment, spleen cells and serum were isolated, and Tregs/Th17 cells were detected by flow cytometry. The expression of forkhead box P3 (Foxp3), retinoid-related orphan receptor gamma-t (RORγt), IL-10, and IL-17 mRNA was measured by real-time PCR, and protein levels were measured by Western blot and enzyme-linked immunosorbent assay.The frequency of Treg cells increased significantly (p0.05) in the FBL-3-inoculated leukemia mouse model compared with control mice, whereas the frequency of Th17 cells did not change. Median survival of mice after treatment with CpG 1826 and tumor vaccine was significantly prolonged compared with that of control mice (p0.05). The frequency of induced Treg cells decreased after treatment with CpG 1826, whereas the frequency of Th17 cells induced by cytokines in vitro and in the murine leukemia model increased following treatment with CpG 1826. Furthermore, after treatment with CpG 1826, the mRNA and protein levels of Foxp3 and IL-10 decreased significantly both in vitro and in vivo (p0.05), whereas those of RORγt and IL-17 increased significantly (p0.05).CpG 1826 may inhibit the differentiation of Treg cells induced by cytokines, promote the differentiation of Th17 cells in vitro and in murine leukemia models, and prolong the median survival of mice with leukemia. |
| Databáze: | OpenAIRE |
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