Multiple gene substitution by Target-AID base-editing technology in tomato
| Autor: | Tohru Ariizumi, Akihiko Kondo, Keiji Nishida, Hiroshi Ezura, Sanae Kishimoto, Johan Hunziker |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Science Biology 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Solanum lycopersicum Cytidine Deaminase Nucleotide Allele Carotenoid Gene Base Pairing Alleles Plant Proteins Genetics chemistry.chemical_classification Gene Editing Multidisciplinary food and beverages Cytidine Cytidine deaminase biology.organism_classification Carotenoids Thymine 030104 developmental biology chemistry Medicine Solanum CRISPR-Cas Systems Plant sciences 010606 plant biology & botany Biotechnology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| Popis: | The use of Target activation-induced cytidine deaminase (Target-AID) base-editing technology with the CRISPR-Cas 9 system fused with activation-induced cytidine deaminase (AID) resulted in the substitution of a cytidine with a thymine. In previous experiments focusing on a single target gene, this system has been reported to work in several plant species, including tomato (Solanum lycopersicum L.). In this research, we used Target-AID technology to target multiple genes related to carotenoid accumulation in tomato. We selected 3 genes, SlDDB1, SlDET1 and SlCYC-B, for their roles in carotenoid accumulation. Among 12 edited T0 lines, we obtained 10 independent T0 lines carrying nucleotide substitutions in the three targeted genes, with several allelic versions for each targeted gene. The two edited lines showed significant differences in carotenoid accumulation. These results demonstrate that Target-AID technology is a highly efficient tool for targeting multiple genes with several allelic versions. |
| Databáze: | OpenAIRE |
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