Hypotensive effect of Eugenia dysenterica leaf extract is primarily related to its vascular action: The possible underlying mechanisms
| Autor: | Victor Hugo Martins de Miranda, Samanta Aparecida-Castro, Hudson Armando Nunes Canabrava, Juliana De Gobbi, Luiz Borges Bispo-da-Silva, Denise Brentan Silva, Patrícia Fidelis-de-Oliveira, Ingrid Beatriz de Melo Morais |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade Federal de Mato Grosso do Sul (UFMS), Universidade Federal de Uberlândia (UFU) |
| Rok vydání: | 2020 |
| Předmět: |
Male
Eugenia dysenterica MALDI-TOF Leaves Mean arterial pressure animal structures Calcium Channels L-Type Carbenoxolone Pharmacology Eugenia Hemolysis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Nifedipine Heart Rate Drug Discovery medicine Animals Arterial Pressure Proanthocyanidins Rats Wistar 030304 developmental biology 0303 health sciences Plant Extracts Chemistry musculoskeletal neural and ocular physiology Calcium channel Heart Calcium Channel Blockers musculoskeletal system Plant Leaves Atropine Mechanism of action 030220 oncology & carcinogenesis Hexamethonium Hypotension medicine.symptom tissues Prodelphinidin medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 0378-8741 |
| Popis: | Made available in DSpace on 2020-12-12T01:09:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-06 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Ethnopharmacological relevance: Eugenia dysenterica (ED) leaves are used in Brazil to treat cardiac diseases; however, there are no scientific data describing the effects of this species on cardiac activity. Aim of the study: To investigate the effect of ED aqueous leaf extract (EDLE) on hear rate (HR) and mean arterial pressure (MAP) of anaesthetised rats and its underlying mechanism of action. Material and methods: EDLE was analysed, and its proanthocyanidin composition was determined. After performing dose-effect curves for EDLE on HR and MAP, EDLE-induced hypotension was evaluated before and after atropine (AT), L-N(ω)-nitro-L-arginine methyl ester (L-NAME), hexamethonium (HXT), indomethacin (IND), carbenoxolone (CBX), or nifedipine (NFD) administration. The effect of proanthocyanidin-depleted extract (EDLE/P-) was also determined and compared to that of the EDLE with proanthocyanidins. Results: EDLE decreased the MAP in a dose-dependent manner; HR was decreased only with the highest and most toxic dose. Only CBX and NFD decreased EDLE-induced hypotension. Five polymeric series of proanthocyanidins were identified, which were mainly constituted by procyanidin and prodelphinidin units with B-type linkage and up to 12 flavan-3-ol units. EDLE/P- induced hypotension did not differ from that induced by EDLE. Conclusions: The cardiovascular effects of EDLE were primarily related to its vascular action. EDLE-induced hypotensive effect appeared to involve L-type calcium channel blockage as well as myoendothelial gap junction signalling. The higher molecular weight proanthocyanidins from EDLE are unlikely to contribute to its cardiovascular effect. São Paulo State University/UNESP Department of Physiology Institute of Biosciences Federal University of Mato Grosso do Sul/UFMS Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM) Federal University of Uberlândia/UFU Department of Pharmacology Institute of Biomedical Sciences Campus Umuarama São Paulo State University/UNESP Department of Physiology Institute of Biosciences FAPESP: 2013/18382-7 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect