Influence of the shared epitope on the elicitation of experimental autoimmune arthritis biomarkers

Autor: David D. Brand, Amanda Gamboa, Indra Sandal, Edward F. Rosloniec, Jiwen Luo, Anastasios Karydis, Amanda Prislovsky
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Knees
Alveolar Bone Loss
Arthritis
Knee Joints
Biochemistry
Arthritis
Rheumatoid
Epitopes
Mice
Skeletal Joints
Medicine and Health Sciences
Enzyme-Linked Immunoassays
Musculoskeletal System
Immune Response
Multidisciplinary
biology
Animal Models
Phenotype
Phenotypes
Experimental Organism Systems
Rheumatoid arthritis
Legs
Medicine
Anatomy
Porphyromonas gingivalis
Research Article
Transgene
Science
Immunology
Mouse Models
Rheumatoid Arthritis
Context (language use)
Research and Analysis Methods
Autoimmune Diseases
Periodontal pathogen
Model Organisms
Immune system
Rheumatology
Genetics
medicine
Animals
Humans
Immunoassays
Skeleton
Correction
Biology and Life Sciences
Proteins
medicine.disease
biology.organism_classification
Arthritis
Experimental
Body Limbs
Immunologic Techniques
Animal Studies
Clinical Immunology
Clinical Medicine
Collagens
Biomarkers
Zdroj: PLoS ONE, Vol 16, Iss 4, p e0250177 (2021)
PLoS ONE
ISSN: 1932-6203
Popis: Our previous studies have shown that inoculation of the oral cavity of “humanized” B6.DR1/4 mice with the periodontal pathogen Porphyromonas gingivalis results in an increase in the percentage of circulating Th17 cells, loss of bone and an exacerbation of experimental autoimmune arthritis. The aim of this study was to assess the role played by the human HLA-DRβ molecule containing the shared epitope supplied as a transgene to I-A˚ (murine class II null) C57BL/6 (B6) mice in driving these findings. We compared various immune response parameters as well as alveolar and peri-articular bone loss between humanized B6.DR1 (or B6.DR4) mice and their WT (B6) counterparts. We found that the presence of the shared epitope in the context of inoculation with P. gingivalis enhanced the percentage of Th17 cells generated, dramatically enhanced bone loss and importantly allowed for the generation of CCP2⁺ ACPAs that are not found in C57BL/6 or DBA/1 arthritic mouse serum. Due to the exceedingly complex nature of environmental factors impacting on genetic elements, it has been difficult to unravel mechanisms that drive autoimmune arthritis in susceptible individuals. The findings in this study may provide one small piece of this puzzle that can help us to better understand part of this complexity.
Databáze: OpenAIRE
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