Acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia Roxb., leaves extract in Wistar rats: safety assessment of a rare mangrove traditionally utilized as pain antidote
| Autor: | Sumanta Mondal, Karipeddi Ramakrishna, Debjit Ghosh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Urinalysis
medicine.medical_treatment lcsh:Medicine lcsh:RX1-681 01 natural sciences Locomotor 03 medical and health sciences 0302 clinical medicine lcsh:Homeopathy Oral administration Diabetes mellitus medicine Neurotoxicity Aegialitis rotundifolia Antidote Mangrove General Environmental Science Acute toxicity medicine.diagnostic_test Traditional medicine biology business.industry Stomach lcsh:R medicine.disease biology.organism_classification Plumbaginaceae 030205 complementary & alternative medicine 0104 chemical sciences 010404 medicinal & biomolecular chemistry Sub-acute toxicity medicine.anatomical_structure Toxicity General Earth and Planetary Sciences business |
| Zdroj: | Clinical Phytoscience, Vol 5, Iss 1, Pp 1-16 (2019) |
| ISSN: | 2199-1197 |
| DOI: | 10.1186/s40816-019-0106-2 |
| Popis: | Background Aegialitis rotundifolia Roxb., (Plumbaginaceae) is a small mangrove tree or shrub traditionally used by the local healers of mangroves for curing pain and inflammation. However, there are no scientific reports of its toxicological properties which would guarantee the safety of its folkloric usage as a potent pain reliever. Therefore, the present study deals in acute and sub-acute (30-day) toxicity studies of Aegialitis rotundifolia ethanolic leaves extract (ARELE) on male and female Wistar rats administered through oral gavage. Methods Acute toxicity study was conducted at a single oral dose of 1000, 1500, and 2000 mg/kg, body weight (b.wt.) for 14 days with a special emphasis on the first four hours after drug administration to find out any mortality and morbidity. In sub-acute toxicity, the extract at the doses of 125, 250 and 500 mg/kg, b.wt., was administered orally for 30 days. Important parameters such as general behaviour, body and organ weight, urinalysis, haematological and biochemical profile, organ macroscopy and microscopy were conducted. Organ sample of liver, kidney, pancreas, heart, lungs, and stomach were taken from both male and female rats, whereas the sample of testis and ovary was taken from male and female rats respectively for organ necropsy and histopathological studies. Neurobehavioral toxicity was conducted by performing a functional observational battery (FOB) and locomotor activity on the initial and final week of the 30-day study period. Results No mortality or any major signs of morbidity was recorded for acute toxicity except for the limit dose (2000 mg/kg, b.wt.) which produced a slight short-term sedative effect. In sub-acute toxicity, no major alteration was observed in the evaluated parameters. However, few minor changes were recorded for high dose (500 mg/kg, b.wt.) group. Conclusion The results of the present study showed that oral administration of Aegialitis rotundifolia ethanolic leaves extract did not produce any severe toxic effects in both acute and sub-acute studies in Wistar rats. Therefore, usage of an appropriate amount of Aegialitis rotundifolia leaves extract preferably at low doses for its traditional use should be considered safe. |
| Databáze: | OpenAIRE |
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